FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_RELA_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_RELA_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4691685
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT1680373.5815916882740426No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1157692.4675356508375987No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA512131.091569446797899No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG496751.058788047364646No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA409310.8724157738637611No Hit
AAAAAAAAAAAAAAAAAAAAATTTT385160.8209417298902207No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT315950.6734254324405837No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC307550.6555214171454392No Hit
AAAAAAAAAAAAAAAAAAAAATTTA259680.5534898442670384No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT245230.5226906750986052No Hit
AAAAAAAAAAAAAAAAAAAAAATTT213800.45569981786927294No Hit
TATTTTTTTTTTTTTTTTTTTTTTT181770.3874301024045732No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG181750.3873874737967276No Hit
AAAAAAAAAAAAAAAAAAAATTTTT173440.3696752872368882No Hit
AAAAAAAAAAAAAAAAAAAAAATTA160040.34111411998034824No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA154320.3289223381365117No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC154010.3282615947149052No Hit
AAAAAAAAAAAAAAAAAAAAAAATT149640.3189472439006455No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG146900.31310712462580076No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT126280.2691570299370056No Hit
AAAAAAAAAAAAAAAAAAAATTTTA123920.2641268542112269No Hit
TTTATTTTTTTTTTTTTTTTTTTTT123600.2634447964856976No Hit
TTATTTTTTTTTTTTTTTTTTTTTT122820.2617822807797199No Hit
TATTTTTTTTTTTTTTTTTTTTTTA120340.25649633340686767No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT107380.22887299552293047No Hit
AAAAAAAAAAAAAAAAAAAAAATAA106970.22799910906209603No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC98360.20964749338457292No Hit
AAAAAAAAAAAAAAAAAAAAATTAA90200.19225502138357542No Hit
TTTTTTTTTTTTTTTTTTTTTTACC90090.19202056404042472No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT85670.18259964170655107No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT80710.1720277469608467No Hit
AAAAAAAAAAAAAAAAAAAAAAATA79370.1691716302351927No Hit
TTATTTTTTTTTTTTTTTTTTTTTA79130.1686600869410457No Hit
TTTATTTTTTTTTTTTTTTTTTTTA78660.16765831465667452No Hit
TTTTATTTTTTTTTTTTTTTTTTTT78210.16669917098014894No Hit
TTTTTATTTTTTTTTTTTTTTTTTT76170.16235105297989955No Hit
TTTTTTTTTTTTTTTTTTTTTTACA75440.1607951087935358No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA72040.15354824545978685No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC70580.15043635708705932No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA67070.14295503641015966No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA64870.13826588954714564No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC64160.13675257396862747No Hit
AAAAAAAAAAAAAAAAAAAAATAAA61920.13197816988992228No Hit
TTTTTTTTTTTTTTTTTTTTTAATT58980.1257117645366217No Hit
TATTTTTTTTTTTTTTTTTTTTTAA58790.12530679276208867No Hit
TTTTTTATTTTTTTTTTTTTTTTTT57530.12262119046781698No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA50500.10763723481009488No Hit
TTTTTATTTTTTTTTTTTTTTTTTA50180.10695517708456558No Hit
TTTTTTTATTTTTTTTTTTTTTTTT48770.10394986023145203No Hit
TTTTATTTTTTTTTTTTTTTTTTTA48750.10390723162360645No Hit
TATTTTTTTTTTTTTTTTTTTTTTG48690.1037793458000697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)