FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_RELA_no_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_RELA_no_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4188820
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2448405.845082863431706No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1654513.9498235780004873No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA726481.7343309094207917No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG670891.601620504103781No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC447591.068534814100391No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT421561.0063932085885763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG274940.6563662320176088No Hit
TATTTTTTTTTTTTTTTTTTTTTTT258890.6180499520151259No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA218780.5222950616163979No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC212850.508138330126384No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA206910.4939577255647175No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT196130.4682225543231745No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG175760.4195931073667524No Hit
AAAAAAAAAAAAAAAAAAAAATTTT175010.4178026269928047No Hit
TTTATTTTTTTTTTTTTTTTTTTTT168280.40173604977057975No Hit
TATTTTTTTTTTTTTTTTTTTTTTA164800.393428220835462No Hit
TTATTTTTTTTTTTTTTTTTTTTTT155760.37184696406147794No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT149250.3563055944156111No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC129120.3082491011788523No Hit
TTTTTTTTTTTTTTTTTTTTTTACC122720.29297033532116445No Hit
TTTTTATTTTTTTTTTTTTTTTTTT110010.26262766125066245No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT109380.26112365773654633No Hit
TTTTTTTTTTTTTTTTTTTTTTACA109330.26100429237828315No Hit
TTTTATTTTTTTTTTTTTTTTTTTT106540.25434370538719736No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT105980.25300681337464964No Hit
TTTATTTTTTTTTTTTTTTTTTTTA103320.24665657631504817No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC102570.24486609594110034No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA98280.23462454820211898No Hit
TTATTTTTTTTTTTTTTTTTTTTTA98150.23431419827063468No Hit
AAAAAAAAAAAAAAAAAAAAAATTT95400.22774910356615943No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA95330.22758199206459098No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA87680.20931909225032347No Hit
AAAAAAAAAAAAAAAAAAAAATTTA84130.20084415181363724No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC83180.1985762100066367No Hit
TTTTTTTTTTTTTTTTTTTTTAATT82720.1974780487106154No Hit
AAAAAAAAAAAAAAAAAAAATTTTT81950.19563982219336232No Hit
TATTTTTTTTTTTTTTTTTTTTTAA80170.1913904154391929No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT79790.19048323871639267No Hit
TTTTTTATTTTTTTTTTTTTTTTTT75100.1792867681113058No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA73250.17487024985556793No Hit
TTTTTATTTTTTTTTTTTTTTTTTA66500.15875592649003775No Hit
TTTTATTTTTTTTTTTTTTTTTTTA65780.1570370653310479No Hit
TTTTTTTATTTTTTTTTTTTTTTTT63940.1526444201469626No Hit
AATTTTTTTTTTTTTTTTTTTTTTT62580.14939768240220397No Hit
TATTTTTTTTTTTTTTTTTTTTTTG60790.1451244025763819No Hit
AAAAAAAAAAAAAAAAAAAAAAATT57230.13662558906804304No Hit
TTTTTTTTATTTTTTTTTTTTTTTT56770.1355274277720217No Hit
TAATTTTTTTTTTTTTTTTTTTTTT52580.12552461074956672No Hit
AAAAAAAAAAAAAAAAAAAAAATTA52040.12423546488032429No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT50170.11977120048128112No Hit
TTTTTTTTTATTTTTTTTTTTTTTT48950.11685868573965937No Hit
ATTTTTTTTTTTTTTTTTTTTTTAA48910.11676319345304884No Hit
TATATTTTTTTTTTTTTTTTTTTTT47430.11322997884845852No Hit
TTTTTTATTTTTTTTTTTTTTTTTA47190.11265702512879522No Hit
TTTTTTTTTTTTTTTTTTTTTAACA46670.11141562540285807No Hit
TTTTTTTTTTTTTTTTTTTTAAATT45730.10917155666751019No Hit
TTTTTTTTTTTTTTTTTTTTTTATT45400.10838374530297316No Hit
TTTTTTTTTTTTTTTTTTTTTTCCC45340.10824050687305733No Hit
TTTATTTTTTTTTTTTTTTTTTTAA44270.10568608820622513No Hit
TTATTTTTTTTTTTTTTTTTTTTAA43950.10492214991334074No Hit
AATTTTTTTTTTTTTTTTTTTTTTA42240.10083985466073977No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)