FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_RELA_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_RELA_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5344795
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT1747023.2686379926638907No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1154532.1601015567482005No Hit
AAAAAAAAAAAAAAAAAAAAATTTT577381.0802659409762208No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA472950.8848795884594264No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA465340.8706414371365039No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG409490.7661472516719537No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT332050.6212586263832383No Hit
AAAAAAAAAAAAAAAAAAAAATTTA310710.5813319313462911No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT296740.555194352636537No Hit
AAAAAAAAAAAAAAAAAAAAAATTT293340.5488330235303693No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC270450.5060063108126691No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG248970.4658176786948798No Hit
AAAAAAAAAAAAAAAAAAAATTTTT237650.44463819472963884No Hit
AAAAAAAAAAAAAAAAAAAAAAATT192500.3601634861580285No Hit
AAAAAAAAAAAAAAAAAAAAAATTA182440.34134143592036736No Hit
TATTTTTTTTTTTTTTTTTTTTTTT178040.3331091276653267No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT165890.3103767310065213No Hit
TTTATTTTTTTTTTTTTTTTTTTTT136980.2562867238126065No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA136950.2562305944381403No Hit
AAAAAAAAAAAAAAAAAAAATTTTA134900.2523950871829509No Hit
TTATTTTTTTTTTTTTTTTTTTTTT133480.2497382967915514No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC128320.2400840443833674No Hit
AAAAAAAAAAAAAAAAAAAAAATAA125610.2350136908899219No Hit
TATTTTTTTTTTTTTTTTTTTTTTA112860.2111587067417927No Hit
AAAAAAAAAAAAAAAAAAAAATTAA111020.20771610510786662No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG103890.194376023776403No Hit
AAAAAAAAAAAAAAAAAAAAAAATA102130.19108310047438676No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT101820.19050309693823617No Hit
TTTTTATTTTTTTTTTTTTTTTTTT89680.16778941007091946No Hit
TTTTATTTTTTTTTTTTTTTTTTTT89500.16745263382412234No Hit
TTATTTTTTTTTTTTTTTTTTTTTA83550.15632030788832874No Hit
TTTATTTTTTTTTTTTTTTTTTTTA82080.1535699685394856No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT78360.14660992610567852No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT77890.14573056590570826No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC76600.14331700280366227No Hit
AAAAAAAAAAAAAAAAAAAAATAAA76400.14294280697388767No Hit
TTTTTTTTTTTTTTTTTTTTTAATT71880.13448598122098226No Hit
TTTTTTTTTTTTTTTTTTTTTTACC71460.13370016997845566No Hit
TTTTTTATTTTTTTTTTTTTTTTTT66490.12440140360855749No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA65760.12303558882988028No Hit
TTTTTTTTTTTTTTTTTTTTTTACA65080.12176332300864673No Hit
AAAAAAAAAAAAAAAAAAATTTTTT62850.11759103950666021No Hit
TTTTTTTATTTTTTTTTTTTTTTTT58730.10988260541330397No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC58570.1095832487494843No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA57560.10769355980912272No Hit
TTTTATTTTTTTTTTTTTTTTTTTA56250.10524257712409923No Hit
TAAAAAAAAAAAAAAAAAAAATTTT56180.10511160858367814No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)