FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_RELA_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_RELA_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4212185
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT1469033.4875723644616747No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA995302.362906662456658No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA411320.9765003199052275No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG389500.9246982266923224No Hit
AAAAAAAAAAAAAAAAAAAAATTTT387090.9189767306041876No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA297470.7062130461981133No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT276470.6563576860940343No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC250420.5945132989173078No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG228030.5413579887872921No Hit
AAAAAAAAAAAAAAAAAAAAATTTA216530.5140562439683918No Hit
AAAAAAAAAAAAAAAAAAAAAATTT189360.44955290425278094No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT187600.4453745502631058No Hit
AAAAAAAAAAAAAAAAAAAATTTTT171360.40681973844928465No Hit
TATTTTTTTTTTTTTTTTTTTTTTT155720.36968936549558007No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT127690.3031443300804689No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA121920.2894459763756815No Hit
AAAAAAAAAAAAAAAAAAAAAAATT121690.28889994147930353No Hit
AAAAAAAAAAAAAAAAAAAAAATTA119790.2843892184222678No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC118220.28066193673829615No Hit
TTTATTTTTTTTTTTTTTTTTTTTT118090.2803533083186042No Hit
TTATTTTTTTTTTTTTTTTTTTTTT105480.25041635160848824No Hit
TATTTTTTTTTTTTTTTTTTTTTTA103190.24497974329237676No Hit
AAAAAAAAAAAAAAAAAAAATTTTA99730.23676547919903804No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG97380.23118642699691488No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT94940.22539370896577432No Hit
AAAAAAAAAAAAAAAAAAAAAATAA79030.18762233852501728No Hit
AAAAAAAAAAAAAAAAAAAAATTAA74830.1776512665042015No Hit
TTTTTATTTTTTTTTTTTTTTTTTT74740.17743760067518402No Hit
TTTTATTTTTTTTTTTTTTTTTTTT72630.1724283240171075No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC71840.1705528128512874No Hit
TTTATTTTTTTTTTTTTTTTTTTTA71500.1697456308305547No Hit
TTATTTTTTTTTTTTTTTTTTTTTA67280.15972707751440168No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT66930.15889615484600036No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT66680.15830263865428512No Hit
TTTTTTTTTTTTTTTTTTTTTTACC66240.1572580501568663No Hit
AAAAAAAAAAAAAAAAAAAAAAATA62250.1477855317370913No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA61230.1453639856748932No Hit
TTTTTTTTTTTTTTTTTTTTTTACA58800.13959500829142119No Hit
TTTTTTATTTTTTTTTTTTTTTTTT57670.1369123151048684No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC55350.13140448484575107No Hit
TTTTTTTTTTTTTTTTTTTTTAATT55200.13104837513072196No Hit
AAAAAAAAAAAAAAAAAAAAATAAA51660.12264418585603434No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA50740.1204600462705223No Hit
TATTTTTTTTTTTTTTTTTTTTTAA49570.11768239049329504No Hit
TTTTTTTATTTTTTTTTTTTTTTTT48020.11400259010466066No Hit
AAAAAAAAAAAAAAAAAAATTTTTT47870.11364648038963152No Hit
TTTTTATTTTTTTTTTTTTTTTTTA47440.11262563253988131No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA46450.11027530842068903No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC45730.10856598178854916No Hit
TTTTATTTTTTTTTTTTTTTTTTTA45130.10714154292843263No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)