FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_NT_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_NT_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4365501
Sequences flagged as poor quality0
Sequence length25
%GC8

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2416675.535836551177058No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1497303.4298468835535716No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA615971.410994980873902No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG573721.3142134201778903No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG571531.3091968138364876No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT403150.9234907975052578No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC341230.7816514072497063No Hit
AAAAAAAAAAAAAAAAAAAAATTTT258190.5914326900852845No Hit
TATTTTTTTTTTTTTTTTTTTTTTT251350.5757643853477528No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA237320.5436260351331955No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT203900.4670712479506934No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA188640.43211535170877297No Hit
TTTATTTTTTTTTTTTTTTTTTTTT175590.40222187556479777No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC166770.3820180089295593No Hit
TTATTTTTTTTTTTTTTTTTTTTTT161910.37088526608973404No Hit
TATTTTTTTTTTTTTTTTTTTTTTA150950.34577932750444906No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG148800.3408543486761313No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT147770.338494940214193No Hit
AAAAAAAAAAAAAAAAAAAAAATTT126600.2900010789139666No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT118230.2708280218009342No Hit
TTTTTATTTTTTTTTTTTTTTTTTT116300.26640699429458387No Hit
TTTTATTTTTTTTTTTTTTTTTTTT114350.261940153031691No Hit
AAAAAAAAAAAAAAAAAAAATTTTT110170.2523650779143104No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC107710.24672998585958403No Hit
TTTATTTTTTTTTTTTTTTTTTTTA98490.2256098440934958No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT96700.22150951288294288No Hit
AAAAAAAAAAAAAAAAAAAAATTTA95730.21928754569063208No Hit
TTTTTTTTTTTTTTTTTTTTTTACC95680.21917301129927586No Hit
TTATTTTTTTTTTTTTTTTTTTTTA93050.2131485023139383No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT91200.2089107298337579No Hit
TTTTTTTTTTTTTTTTTTTTTAATT89220.20437516793605132No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA88280.20222192137855427No Hit
TTTTTTTTTTTTTTTTTTTTTTACA86790.1988087965161387No Hit
TTTTTTATTTTTTTTTTTTTTTTTT79840.18288851611762316No Hit
AAAAAAAAAAAAAAAAAAAAAAATT77830.17828423358510284No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC77410.17732214469771052No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA73940.16937345793758837No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA72460.16598323995344408No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC70830.16224941879523105No Hit
TATTTTTTTTTTTTTTTTTTTTTAA70060.16048558916834518No Hit
TTTTTTTATTTTTTTTTTTTTTTTT68280.15640816483606348No Hit
TTTTTATTTTTTTTTTTTTTTTTTA65310.1496048219895036No Hit
TTTTATTTTTTTTTTTTTTTTTTTA63720.1459626283443756No Hit
AATTTTTTTTTTTTTTTTTTTTTTT59170.13553999873095895No Hit
AAAAAAAAAAAAAAAAAAAAAATTA58660.13437174793912543No Hit
TTTTTTTTATTTTTTTTTTTTTTTT58600.13423430666949795No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA58050.13297442836457946No Hit
TAATTTTTTTTTTTTTTTTTTTTTT53270.12202494055092417No Hit
TATTTTTTTTTTTTTTTTTTTTTTG52980.12136064108105805No Hit
TTTTTTTTTATTTTTTTTTTTTTTT52130.11941355642800218No Hit
TTTTTTTTTTTTTTTTTTTTAAATT51940.11897832574084855No Hit
TATATTTTTTTTTTTTTTTTTTTTT47260.10825790670990569No Hit
TTTTTTTTTTTTTTTTTTTTTTATT46670.10690640089190222No Hit
TTTTTTTTTTTTTTTTTTTTTAAAT45470.10415757549935277No Hit
AAAAAAAAAAAAAAAAAAAAAAATA45230.10360781042084287No Hit
TTTTTTATTTTTTTTTTTTTTTTTA44220.10129421571544708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)