FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_NT_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_NT_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5403901
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT1805513.341123384754828No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1170052.1651951062760033No Hit
AAAAAAAAAAAAAAAAAAAAATTTT546861.0119726471672963No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA452080.8365808329945349No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG385110.712651841697322No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA375240.6943872583898187No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT342680.6341344891403451No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG308510.5709023906988674No Hit
AAAAAAAAAAAAAAAAAAAAAATTT279720.517626063097751No Hit
AAAAAAAAAAAAAAAAAAAATTTTT270050.49973158279546576No Hit
AAAAAAAAAAAAAAAAAAAAATTTA259650.4804862265241351No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT257460.4764335986169991No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC244730.45287654233487995No Hit
TATTTTTTTTTTTTTTTTTTTTTTT185960.34412177425160084No Hit
AAAAAAAAAAAAAAAAAAAAAAATT170570.3156423480000837No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT170340.3152167295440831No Hit
AAAAAAAAAAAAAAAAAAAAAATTA155420.28760704535482795No Hit
TTTATTTTTTTTTTTTTTTTTTTTT153070.2832583350435176No Hit
TTATTTTTTTTTTTTTTTTTTTTTT140500.2599973611655728No Hit
AAAAAAAAAAAAAAAAAAAATTTTA132060.24437901434537754No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA127210.2354040164688435No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT117280.21702840225977493No Hit
TATTTTTTTTTTTTTTTTTTTTTTA115430.21360494946150937No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC111880.20703562111889173No Hit
AAAAAAAAAAAAAAAAAAAAAATAA110190.20390825072480045No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG98350.18199815281590095No Hit
TTTTATTTTTTTTTTTTTTTTTTTT96470.17851918456685273No Hit
TTTTTATTTTTTTTTTTTTTTTTTT95320.17639109228684982No Hit
AAAAAAAAAAAAAAAAAAAAATTAA95100.17598397898111012No Hit
TTTATTTTTTTTTTTTTTTTTTTTA89850.16626877509414031No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT85130.15753434417099796No Hit
TTATTTTTTTTTTTTTTTTTTTTTA83960.15536924159047324No Hit
TTTTTTTTTTTTTTTTTTTTTAATT80780.1494846038075087No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT78600.1454504810506336No Hit
AAAAAAAAAAAAAAAAAAAAAAATA77550.14350744027323964No Hit
AAAAAAAAAAAAAAAAAAATTTTTT74300.1374932664384488No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA72430.13403280333966147No Hit
TTTTTTATTTTTTTTTTTTTTTTTT69510.12862929946348017No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC69400.12842574281061034No Hit
TTTTTTTTTTTTTTTTTTTTTTACC66170.12244857927634129No Hit
AAAAAAAAAAAAAAAAAAAAATAAA62490.11563868398033199No Hit
TTTTTTTATTTTTTTTTTTTTTTTT60100.1112159530679781No Hit
TTTTTTTTTTTTTTTTTTTTTTACA59630.11034621100571605No Hit
TTTTATTTTTTTTTTTTTTTTTTTA57660.10670069640431976No Hit
TTTTTATTTTTTTTTTTTTTTTTTA56770.10505373803110013No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA56330.10423951141962076No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC54380.10063100711874624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)