FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_NT_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_NT_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6189367
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT2394093.868069222587706No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1588042.565755108721134No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA636171.027843396586436No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG569970.9208857707096704No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG560220.9051329481673973No Hit
AAAAAAAAAAAAAAAAAAAAATTTT492780.796171886398076No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT446370.7211884510968569No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA398060.6431352349925282No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC365280.5901734377683533No Hit
TATTTTTTTTTTTTTTTTTTTTTTT270390.4368621217646328No Hit
AAAAAAAAAAAAAAAAAAAAAATTT261710.42283807051674266No Hit
AAAAAAAAAAAAAAAAAAAATTTTT261700.4228219137756737No Hit
AAAAAAAAAAAAAAAAAAAAATTTA235370.38028121454100233No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT217840.3519584474470491No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT215420.34804851610835164No Hit
TTTATTTTTTTTTTTTTTTTTTTTT188430.30444147196312643No Hit
TTATTTTTTTTTTTTTTTTTTTTTT182680.29515134584845265No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA179420.2898842482599594No Hit
TATTTTTTTTTTTTTTTTTTTTTTA176850.285731965805227No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC171390.2769103851815541No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT163240.26374264121032087No Hit
AAAAAAAAAAAAAAAAAAAAAAATT148980.24070312844592995No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG147230.2378756987588553No Hit
AAAAAAAAAAAAAAAAAAAAAATTA141560.2287148265727335No Hit
TTTTTATTTTTTTTTTTTTTTTTTT124900.2017976959517831No Hit
AAAAAAAAAAAAAAAAAAAATTTTA123840.2000850813984693No Hit
TTTTATTTTTTTTTTTTTTTTTTTT118450.1913765979622795No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT113500.18337901113312557No Hit
TTTATTTTTTTTTTTTTTTTTTTTA113340.1831205032760216No Hit
TTATTTTTTTTTTTTTTTTTTTTTA113170.18284583867784865No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT104960.1695811542602014No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA103230.16678603805526476No Hit
AAAAAAAAAAAAAAAAAAAAAATAA101330.1637162572521552No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC99500.1607595736365286No Hit
TTTTTTTTTTTTTTTTTTTTTAATT97480.157495911940591No Hit
TTTTTTTTTTTTTTTTTTTTTTACC97250.15712430689600407No Hit
TTTTTTATTTTTTTTTTTTTTTTTT88510.14300331520169995No Hit
AAAAAAAAAAAAAAAAAAAAATTAA87660.14162999221083514No Hit
TTTTTTTTTTTTTTTTTTTTTTACA86270.13938420520224443No Hit
AAAAAAAAAAAAAAAAAAAAAAATA81720.13203288801585042No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC79150.12788060556111797No Hit
TATTTTTTTTTTTTTTTTTTTTTAA78780.12728280614156506No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA78450.1267496336862881No Hit
TTTTTATTTTTTTTTTTTTTTTTTA76040.12285585908865965No Hit
AAAAAAAAAAAAAAAAAAATTTTTT75750.12238731359765868No Hit
TTTTTTTATTTTTTTTTTTTTTTTT75590.12212880574055474No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA74720.12072316926755192No Hit
TTTTATTTTTTTTTTTTTTTTTTTA72930.1178311126162013No Hit
AATTTTTTTTTTTTTTTTTTTTTTT69250.11188543190281008No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC68740.1110614381082912No Hit
TTTTTTTTATTTTTTTTTTTTTTTT67890.10968811511742639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)