FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_NT_no_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_NT_no_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5152297
Sequences flagged as poor quality0
Sequence length25
%GC6

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT1937413.760284005366927No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1187112.3040403144461585No Hit
AAAAAAAAAAAAAAAAAAAAATTTT541541.0510651851009365No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA480400.9323996656248659No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG418490.8122396670844091No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT355110.6892265721483058No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA315240.6118436107235278No Hit
AAAAAAAAAAAAAAAAAAAAAATTT261010.5065895851888973No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG255870.4966134522136437No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC249280.48382304048077973No Hit
AAAAAAAAAAAAAAAAAAAATTTTT244020.47361400167731016No Hit
AAAAAAAAAAAAAAAAAAAAATTTA219810.4266252508347248No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT209630.4068670730743977No Hit
TATTTTTTTTTTTTTTTTTTTTTTT191220.3711354372622541No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT176070.34173107644997947No Hit
TTTATTTTTTTTTTTTTTTTTTTTT160490.31149213641993073No Hit
AAAAAAAAAAAAAAAAAAAAAAATT150430.2919668644878197No Hit
TTATTTTTTTTTTTTTTTTTTTTTT143840.2791764527549557No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA141420.27447951855259894No Hit
AAAAAAAAAAAAAAAAAAAAAATTA122890.23851497691223933No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC119970.23284760175898245No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT119050.23106199040932615No Hit
TATTTTTTTTTTTTTTTTTTTTTTA116630.22636505620696945No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG108240.21008105705086488No Hit
AAAAAAAAAAAAAAAAAAAATTTTA102330.1986104450112251No Hit
TTTTATTTTTTTTTTTTTTTTTTTT99630.19337006387636427No Hit
TTTTTATTTTTTTTTTTTTTTTTTT96730.1877415063611434No Hit
AAAAAAAAAAAAAAAAAAAAAATAA88250.1712828278338768No Hit
TTTATTTTTTTTTTTTTTTTTTTTA87440.16971071349341857No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT87200.1692449018369865No Hit
TTATTTTTTTTTTTTTTTTTTTTTA84140.16330580321747756No Hit
AAAAAAAAAAAAAAAAAAAAATTAA82800.16070502146906515No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT81110.15742493105502264No Hit
TTTTTTTTTTTTTTTTTTTTTAATT78230.15183519117783775No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC76000.1475070245368231No Hit
TTTTTTATTTTTTTTTTTTTTTTTT73610.14286831679152037No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA70240.13632754478245335No Hit
AAAAAAAAAAAAAAAAAAATTTTTT69330.13456134225181507No Hit
AAAAAAAAAAAAAAAAAAAAAAATA67030.13009731387767437No Hit
TTTTTTTTTTTTTTTTTTTTTTACC65790.12769062031944198No Hit
TTTTTTTTTTTTTTTTTTTTTTACA64180.12456580045754351No Hit
TTTTTTTATTTTTTTTTTTTTTTTT63000.12227555981341913No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA56900.11043618021243728No Hit
TTTTTATTTTTTTTTTTTTTTTTTA56880.11039736257440129No Hit
TTTTATTTTTTTTTTTTTTTTTTTA56790.11022268320323926No Hit
AAAAAAAAAAAAAAAAAAAAATAAA55070.10688436633214274No Hit
TTTTTTTTATTTTTTTTTTTTTTTT54910.10657382522785469No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC54720.10620505766651261No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA54220.10523461671561246No Hit
TAAAAAAAAAAAAAAAAAAAATTTT53270.10339077890890219No Hit
TATTTTTTTTTTTTTTTTTTTTTAA53050.10296378489050612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)