FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_NT_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_NT_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3845573
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT1468443.8185206729920353No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA992672.581331832733379No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA435961.1336672064215136No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG406221.0563315271872358No Hit
AAAAAAAAAAAAAAAAAAAAATTTT295590.7686500815353134No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT280640.7297742105012699No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA262340.682187023884347No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC257750.6702512213394467No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG229030.5955679426707021No Hit
AAAAAAAAAAAAAAAAAAAAATTTA181760.4726473792072079No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT160700.4178831087070769No Hit
TATTTTTTTTTTTTTTTTTTTTTTT158350.4117721858355049No Hit
AAAAAAAAAAAAAAAAAAAAAATTT144260.37513265253318556No Hit
AAAAAAAAAAAAAAAAAAAATTTTT135960.3535493930293353No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA132180.34371990858059387No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC126200.3281695601669764No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT121850.3168578518727898No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG112610.2928302232203107No Hit
TTTATTTTTTTTTTTTTTTTTTTTT104980.27298922683303634No Hit
TTATTTTTTTTTTTTTTTTTTTTTT104330.2712989715706866No Hit
TATTTTTTTTTTTTTTTTTTTTTTA103500.2691406456203016No Hit
AAAAAAAAAAAAAAAAAAAAAAATT100260.26071537323566607No Hit
AAAAAAAAAAAAAAAAAAAAAATTA98790.2568927959500444No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT93840.24402085202907342No Hit
AAAAAAAAAAAAAAAAAAAATTTTA84710.22027926657483812No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC78510.20415683176473312No Hit
TTTTTTTTTTTTTTTTTTTTTTACC72430.18834644407998496No Hit
TTTTTATTTTTTTTTTTTTTTTTTT71560.18608410242114765No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT69570.18090932092564618No Hit
TTTATTTTTTTTTTTTTTTTTTTTA69000.17942709708020105No Hit
AAAAAAAAAAAAAAAAAAAAAATAA67640.17589056299282319No Hit
TTATTTTTTTTTTTTTTTTTTTTTA66180.17209398963431458No Hit
TTTTATTTTTTTTTTTTTTTTTTTT66050.17175593858184462No Hit
AAAAAAAAAAAAAAAAAAAAATTAA64910.16879149089095435No Hit
TTTTTTTTTTTTTTTTTTTTTTACA64090.1666591688676824No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA61840.16080828526724106No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT61600.1601841910165273No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC59690.15521744093793044No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA56410.14668815284484263No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA53800.13990112786833067No Hit
TTTTTTTTTTTTTTTTTTTTTAATT52040.1353244366964299No Hit
AAAAAAAAAAAAAAAAAAAAAAATA51410.13368618928830633No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC50870.13228197722420038No Hit
TATTTTTTTTTTTTTTTTTTTTTAA50730.13191792224461737No Hit
TTTTTTATTTTTTTTTTTTTTTTTT50560.13147585548369514No Hit
AAAAAAAAAAAAAAAAAAAAATAAA44720.11628956204966073No Hit
TTTTTATTTTTTTTTTTTTTTTTTA43920.11420924788061494No Hit
TTTTTTTATTTTTTTTTTTTTTTTT42730.11111478055415928No Hit
TTTTATTTTTTTTTTTTTTTTTTTA42450.11038667059499327No Hit
AAAAAAAAAAAAAAAAAAATTTTTT40210.10456179092166498No Hit
TATTTTTTTTTTTTTTTTTTTTTTG39670.10315757885755908No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA39300.10219543355437538No Hit
AATTTTTTTTTTTTTTTTTTTTTTT38670.10055718614625181No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)