FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n02_CD4_LTBR_NT_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n02_CD4_LTBR_NT_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3881350
Sequences flagged as poor quality0
Sequence length25
%GC7

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTT1573874.054955105826581No Hit
TTTTTTTTTTTTTTTTTTTTTTTTA1024202.6387725920105116No Hit
TTTTTTTTTTTTTTTTTTTTTTTAA441471.1374135287979699No Hit
TTTTTTTTTTTTTTTTTTTTTTTTG407751.0505365401213496No Hit
TTTTTTTTTTTTTTTTTTTTTTTAT284790.7337395493835908No Hit
TTTTTTTTTTTTTTTTTTTTTTTTC280240.7220168240431809No Hit
AAAAAAAAAAAAAAAAAAAAATTTT259890.6695866129053035No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA230300.593350251845363No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG214340.5522305383436176No Hit
TATTTTTTTTTTTTTTTTTTTTTTT173920.44809151455034973No Hit
AAAAAAAAAAAAAAAAAAAAATTTA148740.3832171795895758No Hit
TTTTTTTTTTTTTTTTTTTTTTTAC134000.3452407023329512No Hit
TTTTTTTTTTTTTTTTTTTTTTAAA132100.3403454983446481No Hit
AAAAAAAAAAAAAAAAAAAAAAAAT129740.3342651397065454No Hit
AAAAAAAAAAAAAAAAAAAAAATTT128010.3298079276540379No Hit
TTTTTTTTTTTTTTTTTTTTTTAAT126850.3268192767980213No Hit
AAAAAAAAAAAAAAAAAAAATTTTT119090.306826233140531No Hit
TTTATTTTTTTTTTTTTTTTTTTTT117410.30249784224561044No Hit
TTATTTTTTTTTTTTTTTTTTTTTT109770.28281396936632874No Hit
TATTTTTTTTTTTTTTTTTTTTTTA109720.2826851482087418No Hit
TTTTTTTTTTTTTTTTTTTTTTTGG107540.277068545737952No Hit
ATTTTTTTTTTTTTTTTTTTTTTTT103510.2666855604364461No Hit
AAAAAAAAAAAAAAAAAAAAAATTA81730.21057106419158284No Hit
TTTTTATTTTTTTTTTTTTTTTTTT80270.2068094863900447No Hit
AAAAAAAAAAAAAAAAAAAAAAATT79940.20595926674997103No Hit
TTTTTTTTTTTTTTTTTTTTTTACC78650.20263568088422843No Hit
TTTTTTTTTTTTTTTTTTTTTTTGC78630.20258415242119365No Hit
TTTTATTTTTTTTTTTTTTTTTTTT76110.19609156607881278No Hit
TTTTTTTTTTTTTTTTTTTTTTTCT72090.18573434500882424No Hit
TTTATTTTTTTTTTTTTTTTTTTTA69250.17841730325788707No Hit
AAAAAAAAAAAAAAAAAAAATTTTA68380.17617581511587463No Hit
TTTTTTTTTTTTTTTTTTTTTTTGT67820.1747330181509011No Hit
TTTTTTTTTTTTTTTTTTTTTTACA67360.1735478635011014No Hit
TTATTTTTTTTTTTTTTTTTTTTTA67030.17269764386102773No Hit
ATTTTTTTTTTTTTTTTTTTTTTTA64660.16659152099140764No Hit
TTTTTTTTTTTTTTTTTTTTTTTCC64070.16507143133188193No Hit
TTTTTTTTTTTTTTTTTTTTTTTCA59690.15378669792726757No Hit
TTTTTTATTTTTTTTTTTTTTTTTT58900.1517513236373942No Hit
AAAAAAAAAAAAAAAAAAAAAATAA58430.15054040475607713No Hit
TTTTTTTTTTTTTTTTTTTTTAATT53990.13910108596235846No Hit
TTTTTTTTTTTTTTTTTTTTTAAAA53800.13861156556352816No Hit
TATTTTTTTTTTTTTTTTTTTTTAA52720.13582902855965062No Hit
AAAAAAAAAAAAAAAAAAAAATTAA52340.13484998776199003No Hit
TTTTTTTTTTTTTTTTTTTTTTAAC50980.13134605227562576No Hit
TTTTTTTATTTTTTTTTTTTTTTTT49720.1280997591044353No Hit
TTTTATTTTTTTTTTTTTTTTTTTA46640.12016437579708092No Hit
TTTTTATTTTTTTTTTTTTTTTTTA46290.11926262769397246No Hit
AAAAAAAAAAAAAAAAAAAAAAATA45090.11617091991188633No Hit
AATTTTTTTTTTTTTTTTTTTTTTT43610.11235781364731343No Hit
TTTTTTTTATTTTTTTTTTTTTTTT43080.11099230937689207No Hit
TTTTTTTTTTTTTTTTTTTTTTTGA42730.11009056127378361No Hit
TATTTTTTTTTTTTTTTTTTTTTTG39910.10282504798588121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)