FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n01_CD4_NGFR_RELB_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n01_CD4_NGFR_RELB_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6141684
Sequences flagged as poor quality0
Sequence length50
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA233000.3793747773411983No Hit
CCCTATGGGGATGGTCCACTGTCACTGTTTCTCTGCTGTTGCAAATACAT152970.24906849652310345No Hit
CCTATGGGGATGGTCCACTGTCACTGTTTCTCTGCTGTTGCAAATACATG109080.1776060116411069No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC105470.17172814491921107No Hit
GCTACAACATAGGGTTTTTATACAAGAAAAATAAAGTGAATTAAGCGTGA103860.16910671405432126No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA100790.16410808501381707No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99340.16174716901748773No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT89610.14590460857315354No Hit
CCCATGCCTAGCTGGATTGCAGAGTTAAGTTTATGATTATGAAATAAAAA89420.14559524716673797No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA86300.14051520722980865No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82540.13439310781863736No Hit
GCTCTTTTAAAAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80000.13025743428023975No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTATCCGATCTCGTATGCC78200.12732664200893437No Hit
GTCAGAAATAAGATTTTTTGAGTAACAAATAAATAAGATCAGACTCTGAA76250.12415161704835351No Hit
CCCCTATGGGGATGGTCCACTGTCACTGTTTCTCTGCTGTTGCAAATACA70660.11504987882802176No Hit
GCCACTAAACTGGGTTAAATGTACACTGTTGAGTTTTCTGTACATAAAAA67590.11005124978751757No Hit
GCAAATAAAAAGTGCTATTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAA65450.10656686342052114No Hit
GCTGTTGCAAATACATGGATAACACATTTGATTCTGTGTGTTTTCATAAT64500.10502005638844329No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)