FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n01_CD4_NGFR_RELB_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n01_CD4_NGFR_RELB_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5511410
Sequences flagged as poor quality0
Sequence length50
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGCC193200.35054550468936263No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC183790.3334718338864283No Hit
GGCATGGTTGTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAA154630.28056341299231957No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135700.2462164854365761No Hit
GTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAATCATAAAAC117730.2136113988979227No Hit
CTCAATAAACACATTTTGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA116660.21166997193095777No Hit
GCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCA105790.1919472512478658No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA102730.18639513300589142No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA97850.17754077450235056No Hit
GTCAGAAATAAGATTTTTTGAGTAACAAATAAATAAGATCAGACTCTGAA87130.1580902164781789No Hit
GTCATGTAGGTTGTCTAAAAATAAAATGCATTTAAACTCAAAAAAAAAAA85970.1559854919158618No Hit
GCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGA76200.13825863073151878No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG74500.13517412059708858No Hit
GGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCATTTTTTTTTT74080.1344120651521117No Hit
GCTACAACATAGGGTTTTTATACAAGAAAAATAAAGTGAATTAAGCGTGA67060.12167485271464108No Hit
GCAAATAAAAAGTGCTATTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAA64800.11757426865357504No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64410.11686664574038223No Hit
GCAAATAAATTCCCGTTTCTATCCAAAAGAGCAATAAAAAGTTTTCAGTG64090.1162860320680189No Hit
CCAACTTAGCCAGTTTTATTTGCAAAACAAGGAAATAAAGGCTTACTTCT60340.10948196559501108No Hit
CTTCCTTCAGAGGATTATCCGGGGCATCTACTCAATGAAAAACCATGATA59660.10824816154123901No Hit
CCTCTTCCGCTGTTTTAGCTGAAGAATTAAAAAAAAAAAAAAAAAAAAAA58540.10621601368796733No Hit
CCTCTGGGCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA58210.10561725583834265No Hit
AAACCCAAGAGGGCAGCTGTTGCAGCATCCAGTTCATCTTAAGAATGTCA56530.10256903405843514No Hit
GATTATTGGAAATTTGTTATAATGAATGAAACATTTTGTCATATAAGATT55400.1005187420279021No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)