FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n01_CD4_NGFR_RELA_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n01_CD4_NGFR_RELA_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5902495
Sequences flagged as poor quality0
Sequence length50
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATCTCGTATGCC216340.3665229703710041No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160350.2716647790468268No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC140450.23795022274478841No Hit
CCCTATGGGGATGGTCCACTGTCACTGTTTCTCTGCTGTTGCAAATACAT112370.19037712018392222No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96370.16326993923755972No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA87300.14790355603859046No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA86840.14712422458638255No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTGGTAATGTCGTATGCC82070.1390428962667482No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT79700.13502764508906828No Hit
CCTATGGGGATGGTCCACTGTCACTGTTTCTCTGCTGTTGCAAATACATG77590.1314528856017667No Hit
CCCATGCCTAGCTGGATTGCAGAGTTAAGTTTATGATTATGAAATAAAAA71460.12106744690169158No Hit
GCTACAACATAGGGTTTTTATACAAGAAAAATAAAGTGAATTAAGCGTGA68370.1158323725814253No Hit
GTCAGAAATAAGATTTTTTGAGTAACAAATAAATAAGATCAGACTCTGAA59880.1014486246917617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)