FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n01_CD4_NGFR_RELA_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n01_CD4_NGFR_RELA_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5279043
Sequences flagged as poor quality0
Sequence length50
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC138360.2620929588942541No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA132610.25120083318131714No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACGTTCGAATCTCGTATGCC82420.1561267828278724No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA77320.14646594089118048No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA76370.14466637229513002No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70660.13385001789150042No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT68940.13059185159128273No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54920.10403400767904335No Hit
CCCTATGGGGATGGTCCACTGTCACTGTTTCTCTGCTGTTGCAAATACAT54850.10390140788775543No Hit
CCAACTTAGCCAGTTTTATTTGCAAAACAAGGAAATAAAGGCTTACTTCT53240.10085161268813306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)