FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n01_CD4_NGFR_RELA_no_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n01_CD4_NGFR_RELA_no_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4578383
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC154020.33640698036839645No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA121780.26598910576070195No Hit
GCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCA98910.21603697200518174No Hit
GGCATGGTTGTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAA78510.17147975606234778No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA77340.16892426867739113No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA75490.16488354076100667No Hit
CTCAATAAACACATTTTGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72300.15791601532680863No Hit
GTCATGTAGGTTGTCTAAAAATAAAATGCATTTAAACTCAAAAAAAAAAA71720.15664919252059079No Hit
GGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCATTTTTTTTTT67800.14808721769235994No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG61660.13467636936446778No Hit
GTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAATCATAAAAC57460.125502824905649No Hit
GTAAAGTGGTTGGTTGCAGTTGTGTAGTAGTTAAGGACTATGGCAAGGAG54770.11962738809750081No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52450.11456009687262948No Hit
GTCAGAAATAAGATTTTTTGAGTAACAAATAAATAAGATCAGACTCTGAA51480.11244144493809277No Hit
CTTCCTTCAGAGGATTATCCGGGGCATCTACTCAATGAAAAACCATGATA51160.11174250821742086No Hit
GCTACAACATAGGGTTTTTATACAAGAAAAATAAAGTGAATTAAGCGTGA46210.10093083081952733No Hit
GCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGA45970.1004066282790234No Hit
CCAACTTAGCCAGTTTTATTTGCAAAACAAGGAAATAAAGGCTTACTTCT45930.10031926118893941No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)