FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n01_CD4_NGFR_RELA_no_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n01_CD4_NGFR_RELA_no_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3930871
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC164120.4175156091359905No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA92760.23597823484922298No Hit
GCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCA89180.22687083854952247No Hit
GGCATGGTTGTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAA79220.2015329426989591No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA78850.20059167548362689No Hit
GGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCATTTTTTTTTT73570.1871595379242921No Hit
GTCATGTAGGTTGTCTAAAAATAAAATGCATTTAAACTCAAAAAAAAAAA65250.1659937454065524No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64720.16464544371972523No Hit
CTTCCTTCAGAGGATTATCCGGGGCATCTACTCAATGAAAAACCATGATA62630.15932855593582188No Hit
CTCAATAAACACATTTTGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62000.15772585770431033No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA61830.15729338357834688No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG60690.15439326296894507No Hit
GTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAATCATAAAAC58980.1500430820548423No Hit
GCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGA53520.13615303071507562No Hit
GCAGGAAGGGGGGTCGCCGTGGTCGCCGTCTGTGAACAAGATTCCTCAAA46770.11898126394888053No Hit
GCCACTAAACTGGGTTAAATGTACACTGTTGAGTTTTCTGTACATAAAAA46320.11783647949780086No Hit
GCTTAATGCCAGGAACAGATTTTGCAGTTGGTGGGGTCTCAATAAAAGTT45980.116971531245874No Hit
CCTGATTACGATGCTTTGGATGTTGCCAACAAAATTGGGATCATCTAAAC43850.1115528848440969No Hit
GTCAGAAATAAGATTTTTTGAGTAACAAATAAATAAGATCAGACTCTGAA43660.11106953140919658No Hit
GCTACAACATAGGGTTTTTATACAAGAAAAATAAAGTGAATTAAGCGTGA43030.10946683317768503No Hit
AAACCCAAGAGGGCAGCTGTTGCAGCATCCAGTTCATCTTAAGAATGTCA42060.10699918669424663No Hit
GCTATATACTTTGGTTACCTATGTACCTGTTACCACTTTCAAAAATCTAC40750.10366659195888137No Hit
GTAAAGTGGTTGGTTGCAGTTGTGTAGTAGTTAAGGACTATGGCAAGGAG40340.10262356612567546No Hit
CCAACTTAGCCAGTTTTATTTGCAAAACAAGGAAATAAAGGCTTACTTCT40280.10247092819886483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)