FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n01_CD4_NGFR_NT_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n01_CD4_NGFR_NT_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6335109
Sequences flagged as poor quality0
Sequence length50
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA311660.4919568076887075No Hit
CCCTATGGGGATGGTCCACTGTCACTGTTTCTCTGCTGTTGCAAATACAT183850.2902081084950551No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC133670.21099873735400607No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133530.21077774668123309No Hit
GCTACAACATAGGGTTTTTATACAAGAAAAATAAAGTGAATTAAGCGTGA121260.19140949271748917No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTATCCGATCTCGTATGCC118210.18659505306064977No Hit
CCTATGGGGATGGTCCACTGTCACTGTTTCTCTGCTGTTGCAAATACATG115880.1829171368637856No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA107980.1704469489001689No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA100140.15807147122488344No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT89680.14156031095913268No Hit
GTCAGAAATAAGATTTTTTGAGTAACAAATAAATAAGATCAGACTCTGAA84660.1336362168354167No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCTATCCGCTCTCGTATGCC83210.13134738486741113No Hit
GCTCTTTTAAAAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83060.13111060914658296No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78390.12373899170479939No Hit
CCCATGCCTAGCTGGATTGCAGAGTTAAGTTTATGATTATGAAATAAAAA77090.12168693545762196No Hit
CCTCTGGGCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71370.11265788797004124No Hit
GCAAATAAAAAGTGCTATTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAA71060.11216855148032971No Hit
CTTAAGCAGCATGGAAAAATGGTTGATGGAAAATAAACATCAGTTTCTAA70540.11134772898145874No Hit
CCAACTTAGCCAGTTTTATTTGCAAAACAAGGAAATAAAGGCTTACTTCT69560.10980079427204804No Hit
GCCACTAAACTGGGTTAAATGTACACTGTTGAGTTTTCTGTACATAAAAA67450.10647014913239851No Hit
GTTTATTGTTGGACAACATTGTTTCACACATACATCAAACAGGCCAAAAA66390.10479693403854615No Hit
GTTTATTGTTGGACAACATTGTTTCACACATACATCAAACAGGAATTTTA66000.10418131716439291No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)