FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n01_CD4_NGFR_NT_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n01_CD4_NGFR_NT_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7579187
Sequences flagged as poor quality0
Sequence length50
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACGTCTACCAATCTCGTATGCC425070.5608385173765998No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA247680.32678966754613653No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC206220.27208723046416455No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA142520.18804127672268806No Hit
CCCTATGGGGATGGTCCACTGTCACTGTTTCTCTGCTGTTGCAAATACAT140270.18507262058582272No Hit
GCTACAACATAGGGTTTTTATACAAGAAAAATAAAGTGAATTAAGCGTGA131310.17325077214746118No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121980.16094074469992628No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA121880.16080880442717668No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTACCACTCTCGTATGCC119890.15818319299946024No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTCTACCAGTCTCGTATGCC119030.15704850665381392No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT112280.1481425382432179No Hit
GTCAGAAATAAGATTTTTTGAGTAACAAATAAATAAGATCAGACTCTGAA107420.1417302409875888No Hit
CCCATGCCTAGCTGGATTGCAGAGTTAAGTTTATGATTATGAAATAAAAA101280.13362910824076513No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95790.1263855872668137No Hit
GGCATGGTTGTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAA86090.11358738081010536No Hit
CCTATGGGGATGGTCCACTGTCACTGTTTCTCTGCTGTTGCAAATACATG84860.11196451545528564No Hit
GCCACTAAACTGGGTTAAATGTACACTGTTGAGTTTTCTGTACATAAAAA81100.10700356119990179No Hit
GTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAATCATAAAAC76540.10098708476252137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)