FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n01_CD4_NGFR_NT_no_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n01_CD4_NGFR_NT_no_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5671399
Sequences flagged as poor quality0
Sequence length50
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA187520.33064152248854295No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC184630.3255457780346613No Hit
GGCATGGTTGTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAA168060.2963290010101564No Hit
CTCAATAAACACATTTTGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA134550.2372430506123798No Hit
GTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAATCATAAAAC122410.2158373974393267No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA117220.20668621622284025No Hit
GCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCA108790.1918221588712062No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA108240.19085238051493114No Hit
GTCATGTAGGTTGTCTAAAAATAAAATGCATTTAAACTCAAAAAAAAAAA97260.17149207805693092No Hit
GTCAGAAATAAGATTTTTTGAGTAACAAATAAATAAGATCAGACTCTGAA94850.16724268562307115No Hit
GCTACAACATAGGGTTTTTATACAAGAAAAATAAAGTGAATTAAGCGTGA92650.1633635721979709No Hit
GGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCATTTTTTTTTT88370.1558169333527759No Hit
CCTCTGGGCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78000.13753220325355348No Hit
GCTTAGAATTTGGGGGAAAATTTAGAAATATAATTGACAGGATTATTGGA71210.12555984863699415No Hit
GCTTAATGCCAGGAACAGATTTTGCAGTTGGTGGGGTCTCAATAAAAGTT70110.12362029192444404No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69600.1227210429031708No Hit
CTTCCTTCAGAGGATTATCCGGGGCATCTACTCAATGAAAAACCATGATA69090.12182179388189757No Hit
CAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGCG67540.11908878215057694No Hit
GCAAATAAAAAGTGCTATTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAA67230.11854217980431284No Hit
CCAACTTAGCCAGTTTTATTTGCAAAACAAGGAAATAAAGGCTTACTTCT66560.1173608134430323No Hit
GCAAATAAATTCCCGTTTCTATCCAAAAGAGCAATAAAAAGTTTTCAGTG62260.10977890993033643No Hit
GCCACTAAACTGGGTTAAATGTACACTGTTGAGTTTTCTGTACATAAAAA62040.10939099858782639No Hit
CCCAAAGCTGGTTTCAAGCCAACCCCATGGCCTCCATGACTTTTTCAAAA61110.10775119154903402No Hit
GCCATGAGAGGTTGATGAGTTAATTAAAGGAGAAGATTCCTAAAATTTGA61030.10761013287903037No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57410.10122722806136546No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)