FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n01_CD4_LTBR_RELA_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n01_CD4_LTBR_RELA_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5751953
Sequences flagged as poor quality0
Sequence length50
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATCTCGTATGCC295830.5143122692414211No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTCTCGTATGCC255130.44355369384972376No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA194800.3386675795160357No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126940.22069025946491566No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC118140.205391108028873No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGATGTCGTATGCC91660.15935457052587182No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA89850.15620781324186758No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA79440.13810961250900347No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT77100.13404142905896482No Hit
GCTACAACATAGGGTTTTTATACAAGAAAAATAAAGTGAATTAAGCGTGA76760.1334503254807541No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAACTGCTGTCGTATGCC75760.13171178554484017No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68330.11879443382099958No Hit
CCCATGCCTAGCTGGATTGCAGAGTTAAGTTTATGATTATGAAATAAAAA67720.11773392446009208No Hit
GTCAGAAATAAGATTTTTTGAGTAACAAATAAATAAGATCAGACTCTGAA62820.10921507877411375No Hit
CTACCTGCACGACAACACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57560.10007035871120644No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)