FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n01_CD4_LTBR_RELA_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n01_CD4_LTBR_RELA_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4691685
Sequences flagged as poor quality0
Sequence length50
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA199210.4246022484459208No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA149440.31852095782218964No Hit
GCTACAACATAGGGTTTTTATACAAGAAAAATAAAGTGAATTAAGCGTGA122190.260439479632584No Hit
GTTTATTGTTGGACAACATTGTTTCACACATACATCAAACAGGAATTTTA117170.24973969906334292No Hit
TCAATAAAGTACCCTGTGCTCAACCAAAAAAAAAAAAAAAAAAAAAAAAA104900.2235870481500783No Hit
CCTCTGGGCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA99610.2123117813749218No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT91520.19506850950138382No Hit
GATACAGGCATGGCAGAAGAATGGGAATATTTTATACTGACAGAAATCAG87830.1872035313538739No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78320.16693362832329964No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70930.15118235772435704No Hit
GTCAGAAATAAGATTTTTTGAGTAACAAATAAATAAGATCAGACTCTGAA68700.14642926794957462No Hit
CCCGTGTGTGGTTGGAAAACTTTTGTTTTTTGGGGTTTTTTTTTTCTGAA65750.14014154829235126No Hit
GCAAATAAATTCCCGTTTCTATCCAAAAGAGCAATAAAAAGTTTTCAGTG58810.12534942136993424No Hit
GTCATTGAAGAGTATTTCAAATGCAAGAAATGAAGAAATAAATCTTTGGC58590.12488050668363285No Hit
GTCTTCTGGTTTGTTGAAACAGTAAGTCAGACAAGTAACTGTATGAGTTA56840.12115050349714443No Hit
CTCAATAAACACATTTTGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51110.10893740734938513No Hit
ACCAGAAGGTTGGGGAGAAATAAACGGTTTAGAGACTAAAAAAAAAAAAA50200.10699780569241116No Hit
CCCCTGCCCTCTCCCTGAAATAAAGAACAGCTTGACAGAAAAAAAAAAAA49440.10537791859427904No Hit
CCCTGATACAGGCATGGCAGAAGAATGGGAATATTTTATACTGACAGAAA48490.10335305972161386No Hit
CCGCAGTCCCTCTCCCAGGAGGACCCTAGAGGCAATTAAATGATGTCCTG48220.10277757351569851No Hit
CCCTATGGGGATGGTCCACTGTCACTGTTTCTCTGCTGTTGCAAATACAT47220.10064614312341941No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)