FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n01_CD4_LTBR_NT_stim_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n01_CD4_LTBR_NT_stim_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5403901
Sequences flagged as poor quality0
Sequence length50
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA274660.5082624570657382No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC185000.34234527982655494No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117640.21769458766916716No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC101270.18740165669208225No Hit
GCTACAACATAGGGTTTTTATACAAGAAAAATAAAGTGAATTAAGCGTGA91330.16900753733275276No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA88720.1641776931146592No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA87570.1620496008346563No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81280.15040986132055342No Hit
CCCATGCCTAGCTGGATTGCAGAGTTAAGTTTATGATTATGAAATAAAAA75610.13991744112262605No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT75080.1389366681587986No Hit
GTCAGAAATAAGATTTTTTGAGTAACAAATAAATAAGATCAGACTCTGAA73420.13586481321549007No Hit
GCAAATAAAAAGTGCTATTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAA58000.10732987151319019No Hit
GTTTATTGTTGGACAACATTGTTTCACACATACATCAAACAGGAATTTTA57880.10710780971005945No Hit
CTACCTGCACGACAACACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56300.10418399596883808No Hit
CCTCTGGGCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54470.1007975534710943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)