FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n01_CD4_LTBR_NT_stim_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n01_CD4_LTBR_NT_stim_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6189367
Sequences flagged as poor quality0
Sequence length50
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA335780.5425110516148097No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCATCTCGTATGCC305530.4936369098810912No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137770.22259142170758334No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC132540.21414144612849745No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA111120.17953370675870406No Hit
GCTACAACATAGGGTTTTTATACAAGAAAAATAAAGTGAATTAAGCGTGA107310.1733779884114159No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA101180.16347390613612023No Hit
TCTGGTTAGATTGTTTTCACTTGGTGATCATGTCTTTTCCATGTGTACCT98240.15872382426183484No Hit
GTCAGAAATAAGATTTTTTGAGTAACAAATAAATAAGATCAGACTCTGAA84430.13641136484554883No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCCATCCCTCTCGTATGCC83140.1343271452476481No Hit
TCTATTGATGAGGGTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79260.12805832971287692No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74990.12115940127641485No Hit
CTACCTGCACGACAACACATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74930.12106246083000087No Hit
CCCATGCCTAGCTGGATTGCAGAGTTAAGTTTATGATTATGAAATAAAAA72990.11792805306261528No Hit
CCAACTTAGCCAGTTTTATTTGCAAAACAAGGAAATAAAGGCTTACTTCT71770.11595693065219756No Hit
CTTAAGCAGCATGGAAAAATGGTTGATGGAAAATAAACATCAGTTTCTAA67290.1087187106532865No Hit
GGCATGGTTGTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAA65000.105018816948486No Hit
CCTCTGGGCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA64910.10487340627886503No Hit
GCCACTAAACTGGGTTAAATGTACACTGTTGAGTTTTCTGTACATAAAAA63730.10296691083272327No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)