FastQCFastQC Report
Fri 3 Sep 2021
HLWNJBGXJ_n01_CD4_LTBR_NT_no_stim_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWNJBGXJ_n01_CD4_LTBR_NT_no_stim_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5152297
Sequences flagged as poor quality0
Sequence length50
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA256630.498088522459012No Hit
TCAGAAGGAAAGGAGAATGTTTTGTGGACCACTTTGGTTTTCTTTTTTGC129400.2511501180929593No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCAATATCTCGTATGCC117720.22848061747993176No Hit
CTTTTATAACATGTTCAATAAAAAGCTGAACTTTAAAAAAAAAAAAAAAA107840.20930470429014478No Hit
GGCATGGTTGTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAA101360.1967277895664788No Hit
CTCAATAAACACATTTTGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95630.18560653626916304No Hit
GGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCATTTTTTTTTT85000.16497496165302583No Hit
GCTGGCCCAAGGTGTCCTGCAGGCTGTAATGCAGTTTAATCAGAGTGCCA83640.1623353622665774No Hit
GTGGTTAATCTGGTTTATTTTTGTTCCACAAGTTAAATAAATCATAAAAC83220.1615201918678213No Hit
CTTTGGTTTTCTTTTTTGCGTGTGGCAGTTTTAAGTTATTAGTTTTTAAA81220.15763842806422068No Hit
GTATAAGAGACAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80720.15666798711332053No Hit
GCTACAACATAGGGTTTTTATACAAGAAAAATAAAGTGAATTAAGCGTGA76350.1481863332024532No Hit
GTCATGTAGGTTGTCTAAAAATAAAATGCATTTAAACTCAAAAAAAAAAA70980.13776379738978556No Hit
GTCAGAAATAAGATTTTTTGAGTAACAAATAAATAAGATCAGACTCTGAA67830.1316500193991146No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63120.12250846564163519No Hit
GAGTGAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62140.12060640137787088No Hit
CCTCTGGGCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61830.12000472798831278No Hit
GCTTGTTATCAGCTTTCAGGGCCATGGTTCACATTAGAATAAAAGGTAGT60960.11831616073374651No Hit
CCAACTTAGCCAGTTTTATTTGCAAAACAAGGAAATAAAGGCTTACTTCT57140.11090199186886936No Hit
GCAAATAAAAAGTGCTATTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAA56200.10907756288117706No Hit
GCCATGAGAGGTTGATGAGTTAATTAAAGGAGAAGATTCCTAAAATTTGA52040.10100349416968782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)