FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_9R_2-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_9R_2-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4838099
Sequences flagged as poor quality0
Sequence length101
%GC28

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG488001.008660633029626No Hit
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG478920.9898929310871895No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG372810.7705712512290468No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG333760.6898577313114097No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA251610.5200596349930003No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC222630.46016007526923275No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT212090.43837465913781426No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT197840.40892094188233846No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG168960.34922807491124097No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA168910.34912472853490595No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG160030.3307704120978095No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG141350.2921602058990525No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC133910.2767822651004041No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA125770.2599574750330657No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT115840.23943288469293417No Hit
TTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCA97680.20189748080806116No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA86560.1789132467111566No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT85790.17732171251559756No Hit
TTTTTTTTTTTTTTTTTTGTAAAAACCCATCTCATAAATAACGTCATGCA84650.17496541513515948No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC72120.14906681322560783No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG68180.1409231187704096No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC67240.13898020689531157No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT64590.13350284894955644No Hit
TTTTTTTTTTTTTTTTTTGTTTAGTACGAGATGGCTTCACACCTCGCGGT62590.12936899389615633No Hit
TTTTTTTTTTTTTTTTTTGTTTCGTTTAGTACGAGATGGCTTCACACCTC61530.12717805071785426No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC52030.10754223921420375No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)