FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_9R_1-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_9R_1-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9105547
Sequences flagged as poor quality0
Sequence length101
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG1124141.234566138640545No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG1091611.198840662730092No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG1039281.1413701999451542No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG951661.0451431418672597No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG601440.6605204497873659No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT598500.657291648706003No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT451040.4953464080741113No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT406790.4467496571046199No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG402510.44204922559841814No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC340740.3742114559399891No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG256110.2812681105264736No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA170320.18705081638697818No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG163950.18005508071069207No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC152570.16755720441616523No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC145320.1595950248787909No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC139450.15314840503266855No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA138960.15261027151910808No Hit
TTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC138650.15226981970440656No Hit
TTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCG127560.1400904305913747No Hit
TTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC118620.13027223954804693No Hit
TTTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACG118350.1299757169997585No Hit
TTTTTTTTTTTTTTTTTTGAATATTTGCTACTACCACCAAGATCTGCACC111350.12228809537746606No Hit
TTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACGG104220.11445770363933105No Hit
TTTTTTTTTTTTTTTTTTAAAATCCCAAATCCGCTCTCGCTCGCCGCTAC99960.10977923676633594No Hit
TTTTTTTTTTTTTTTTTTCTTAAAATCCCAAATCCGCTCTCGCTCGCCGC99170.10891163375467722No Hit
TTTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGG96180.10562792109029805No Hit
TTTTTTTTTTTTTTTTTTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGT94550.10383780348396422No Hit
TTTTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAG92740.10185000417877146No Hit
TTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC92400.10147660541426012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)