FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_7R_2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_7R_2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701533
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG582093.4209739099976315No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG500622.9421703840007805No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG440512.5889007148259835No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG390622.2956945295800906No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT231661.3614781494099732No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT201311.183109584122083No Hit
TTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTA193981.140030784004777No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG156140.9176430900840594No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC147490.8668065797137051No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG136970.8049799798181992No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG131150.7707755300661226No Hit
TTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAA129840.7630765903452945No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT120720.7094778649605973No Hit
TTTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAA67740.3981115852587049No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC66910.39323363108443976No Hit
TTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAAT65930.38747411892687356No Hit
TTTTTTTTTTTTTTTTTCGTTTAGTACGAGATGGCTTCACACCTCGCGGT64800.3808330487860065No Hit
TTTTTTTTTTTTTTTTTTGTTTAGTACGAGATGGCTTCACACCTCGCGGT59830.3516240942726353No Hit
TTTTTTTTTTTTTTTTTTGTTTCGTTTAGTACGAGATGGCTTCACACCTC58520.3439251545518071No Hit
TTTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGT57860.34004629942528297No Hit
TTTTTTTTTTTTTTTTTTCGTTTAGTACGAGATGGCTTCACACCTCGCGG57820.33981121729640273No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC56740.33346399981663594No Hit
TTTTTTTTTTTTTTTTTTGTGACACCCAGGCAGACGTGCCCTCGGCCAGA53640.3152451348284165No Hit
TTTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGG48030.28227486625296133No Hit
TTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC47860.28127576720522024No Hit
TTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAAT45480.2672883805368453No Hit
TTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCG36660.21545277111874997No Hit
TTTTTTTTTTTTTTTTTTGACTTTCCAGCACCGGGCAGGTGTCAGACTCT35740.2100458821545042No Hit
TTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC34460.20252325403033616No Hit
TTTTTTTTTTTTTTTTCGTTTAGTACGAGATGGCTTCACACCTCGCGGTG32710.1922384108918252No Hit
TTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAATC32690.19212086982738505No Hit
TTTTTTTTTTTTTTTTTTCTCCGCTTATTGATATGCTTAAACTCAGCGGG32430.19059283598966342No Hit
TTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTCT32210.18929988428082206No Hit
TTTTTTTTTTTTTTTTTGTGACACCCAGGCAGACGTGCCCTCGGCCAGAA29320.17231520046922394No Hit
TTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGGG28870.1696705265193211No Hit
TTTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGT27330.16061986455743144No Hit
TTTTTTTTTTTTTTTTTGTTTCGTTTAGTACGAGATGGCTTCACACCTCG26690.15685855049534744No Hit
TTTTTTTTTTTTTTTTTTCTCTTTACCTCGCGGTACTTGTTCGCTATCGG26540.1559769925120465No Hit
TTTTTTTTTTTTTTTTTGTTTAGTACGAGATGGCTTCACACCTCGCGGTG26280.15444895867432487No Hit
TTTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATC25370.14910084024229914No Hit
TTTTTTTTTTTTTTTTTGACTTTCCAGCACCGGGCAGGTGTCAGACTCTA24080.14151944158591107No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTTTTCGCTATCGG23810.13993263721596938No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22180.13035304046409912No Hit
TTTTTTTTTTTTTTTTTTTGTTTAGTACGAGATGGCTTCACACCTCGCGG20630.12124360796998941No Hit
TTTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTAT19490.11454376729690226No Hit
TTTTTTTTTTTTTTTTTTTGTGACACCCAGGCAGACGTGCCCTCGGCCAG19440.11424991463580195No Hit
TTTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACG19120.11236925760475995No Hit
TTTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATC18990.11160524068589914No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTCCTTGTTCGCTATCGG18040.10602204012499317No Hit
TTTTTTTTTTTTTTTTTTTGTTTCGTTTAGTACGAGATGGCTTCACACCT18000.10578695799611292No Hit
TTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC17850.10490540001281197No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)