FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_7R_1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_7R_1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7994359
Sequences flagged as poor quality0
Sequence length101
%GC28

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG1247931.5610132094393059No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG1115291.3950962172201673No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG949701.1879626621721642No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG672730.8415058668243445No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT533030.6667576474861837No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG405270.5069449595646129No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT389950.4877814468927402No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC359380.449541983290968No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT352560.44101096785871136No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG347200.43430624018761227No Hit
TTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338730.4237112694088419No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG268360.3356867010851026No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA259600.3247289745181571No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC248320.3106190252401725No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA205600.25718134499589024No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC157860.19746423697009352No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC156010.1951501052179418No Hit
TTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125460.1569356592567334No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA124720.15601000655587272No Hit
TTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCG110200.13784719950655205No Hit
TTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC107020.13386939465690745No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99790.12482551759309282No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA98790.1235746355649027No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT96610.12084771274344822No Hit
TTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC93340.11675732851126651No Hit
TTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTC92860.11615690513773526No Hit
TTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTCT86980.1088017188119773No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT81990.10255981749130855No Hit
TTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81930.10248476456961716No Hit
TTTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATC80550.10075854737071478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)