FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_5R_1-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_5R_1-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6790772
Sequences flagged as poor quality0
Sequence length101
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG1876972.764000911825636No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG1417082.086773050251135No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG1343761.9788029991288179No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT678840.9996507024532704No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC598290.8810338500541618No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG571240.8412003819300663No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG546350.8045477009094106No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT532830.7846383297804728No Hit
TTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480890.7081521806357216No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG342550.504434547353379No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT333650.49132852641790953No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA294690.43395655162623625No Hit
TTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTA290700.42808093100460454No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC272020.40057301290633823No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG245030.3608278999795605No Hit
TTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTC225680.33233334884457905No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA222720.327974492443569No Hit
TTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAA209240.3081240247795096No Hit
TTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176340.25967592491693137No Hit
TTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA159210.23445051608270753No Hit
TTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTCG157990.23265396040391284No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC156360.23025364421011338No Hit
TTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC152260.22421603906006565No Hit
TTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAA149470.22010752238478923No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC145920.21487983987682108No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA143360.21111001812459612No Hit
TTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135650.1997563752692625No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123280.18154047875558182No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT119080.17535561494333782No Hit
TTTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATC117480.1729994763481972No Hit
TTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCG112950.16632865895070548No Hit
TTTTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAG110130.16217596467677017No Hit
TTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAA109620.16142494549956912No Hit
TTTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGT105420.1552400816873251No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC104550.1539589313262174No Hit
TTTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTAT101430.14936446106569326No Hit
TTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAAA101030.1487754264169081No Hit
TTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC98670.14530012198907577No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT94530.1392036133741495No Hit
TTTTTTTTTTTTTTTTTTGACTTTCCAGCACCGGGCAGGTGTCAGACTCT93780.13809917340767736No Hit
TTTTTTTTTTTTTTTTTTGAATATTTGCTACTACCACCAAGATCTGCACC93490.13767212328730813No Hit
TTTTTTTTTTTTTTTTTTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGT93420.13756904222377073No Hit
TTTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGG92130.13566940548143863No Hit
TTTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGG91820.13521290362863014No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG91570.1348447569731394No Hit
TTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAAT90520.13329854102007843No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA90450.13319545995654103No Hit
TTTTTTTTTTTTTTTTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTT90180.13279786156861104No Hit
TTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC89960.13247389251177924No Hit
TTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCA89110.1312221938831108No Hit
TTTTTTTTTTTTTTTTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGG88520.1303533677761527No Hit
TTTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAA88230.12992631765578347No Hit
TTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTCT82460.12142949284705774No Hit
TTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAAT82030.12079628059961371No Hit
TTTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTT78600.11574530848628109No Hit
TTTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACG74780.11012002759038295No Hit
TTTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGT71040.10461255362424185No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)