FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_5G_1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_5G_1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5360992
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2055063.8333577069318516No Hit
TTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAA447960.8355916218490905No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA355300.6627504760313017No Hit
TTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAAA336620.6279061785579982No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG334260.6235040082134052No Hit
TTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA286910.5351808023589664No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC268740.5012878213584352No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA248620.4637574538443631No Hit
TTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAA236480.44111239113955025No Hit
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG172560.3218807265521008No Hit
TTTTTTTTTTTTTTTTTGCCTTGCGACCATACTCCCCCCGGAACCCAAAA169330.31585572222454356No Hit
TTTTTTTTTTTTTTTTTTGCCTTGCGACCATACTCCCCCCGGAACCCAAA165440.3085996024616339No Hit
TTTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCA157670.29410601619998683No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG155310.28970384585539394No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT152270.2840332535471047No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA142670.26612611994198093No Hit
TTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTC140280.26166798980487194No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG127790.23837006285403897No Hit
TTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAAAA123340.2300693602974972No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC113320.21137878959714917No Hit
TTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTCG113220.21119225695542915No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT111950.20882329240558464No Hit
TTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAA106570.19878783628104651No Hit
TTTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCC102790.191736902424029No Hit
TTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCA101270.1889016062698844No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA100810.1880435561179722No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG89940.16776745796300388No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84380.15739624308336966No Hit
TTTTTTTTTTTTTTTTGCCTTGCGACCATACTCCCCCCGGAACCCAAAAA77510.14458145059720293No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT68210.1272339149172392No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT65570.12230945317583015No Hit
TTTTTTTTTTTTTTTTTTGGCCTTGCGACCATACTCCCCCCGGAACCCAA64780.12083584530624183No Hit
TTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCAT58160.10848738442437518No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57870.10794643976338708No Hit
TTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCAT56010.10447693262739433No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)