FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_4G_1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_4G_1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104913
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2341217.540340099706497No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG385241.2407432994096774No Hit
GTGAGATGTTGGATTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTT141540.45585818346601015No Hit
CTGCTGTTGCTGTTGCTGGCCTTGTTGCATCATAGGCATGAAGAAGTTTG111670.3596558100017617No Hit
CCAAGATTCCCTAACGGCGGGCTCATGTCTCAGTACCTAGACTCTTTGCC109390.35231260908115625No Hit
CCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCTAGGTTTT104120.3353395087076514No Hit
GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG97910.3153389483054759No Hit
CTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTACGCCCACGACG91430.2944687983205971No Hit
CACGCCGTTAGGCGCGTGCTGCAGACCACGATCACGGCAGCGACGTCTCC85190.2743716168536767No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79820.2570764462643559No Hit
CAAGAAGTTGTGCAACAACTGCACAAGGTTCTTCGACCATTTCTTCTTCG77190.24860599958839427No Hit
CTTAGCTGGTGGTCTGGGCTGTTTCCCTTTTGACTACGGATCTTATCACT73120.2354977418046818No Hit
GTGGGCTTACTACTTAGATGCTTTCAGCAGTTATCCGCTCCGCACTTGGC71250.22947502876892203No Hit
GCCTACAGAGATTCCAGACAATGGTCTTCTTTGTGATTTACTTTGGTCTG71150.229152958553106No Hit
GCGTTAGCTACAGCACTGCACGGGTCGATACGCACAGCGCCTAGTATCCA66040.2126951705249068No Hit
GGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGGGA65490.21092378433791864No Hit
AAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCT64500.20773528920133996No Hit
TTCCTGCAGATATCAGGCCAGAACTGTTCAGCTTCAGCTTCAGAGACAAA61620.19845966698583828No Hit
CCACGTTCCCCGCCCATCCAAGACTCATATCGTAACCGCGGTGCTTGAGT58810.1894094939214078No Hit
GCCCCAGCCAAACTCCCCACCTGACAATGTCCTCCGCCCGGATCGACCCG57630.18560906537477861No Hit
CCTCGATCGATTAGTATCAGTCAGCTACACATGTCGCCACGCTTCCACCT55290.1780726223246835No Hit
CGCGAATCCTCTTGAAAGAGAGGGGTGCCTTCGGGAACGCGGACACAGGT54960.17700979061249061No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54860.17668772039667457No Hit
GTGGTCCACGAGCTCCATCTTCGATTTCAAATGTAACTTCTTGACCTTCT53590.17259742865581096No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA52140.16792741052647853No Hit
CGGAGACACCAGCCCAACTGTTACGAATGTCGCCGGAGATAGCTAAACCG50530.16274208005184043No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG50140.1614860062101579No Hit
TACCAAGCTTGAGTACGGTAGGGGCAGAGGGAATTTCCGGTGGAGCGGTG50030.16113172897276026No Hit
TCCAAATCGGTGACGACTCGTGCCTTTTCAACGGAACTTCCATCGACGCT49880.16064862364903623No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48650.15668715999449906No Hit
AGACTTTGTTTGTGTAGCAAAAAAAAACGCGATTTTTTTTCTGGGTTTCT45710.14721829564950775No Hit
CGCATATTCCTCGTACTCTTCCCTATGCAAAATCGTCGCATATTCCTCGT41140.13249968678671512No Hit
AGATGATTGAAAGATGGTTTCGGCTATCGCTTACAGATGGGCCCGCGGTG39160.1261226965135577No Hit
TTTTTTTTTTTTTTTTTTAAGCAATATTAAAAAGTTTAAAAGGTGATATA38890.12525310693085442No Hit
CCGTCGCTAAACCGGAAGATTAGAAAGACATTGAAGGATACGGAACAAAG38500.1239970330891719No Hit
GCGAAGATCCAAGACAAGGAAGGAATCCCACCGGACCAGCAGCGATTGAT38390.12364275585177427No Hit
CGAGCCTAGACATATATGTTCCACGAGATGAGAGATTTGGACACTTGAAG38000.12238668201009174No Hit
TTTTTTTTTTTTTTTTTTGAAACAAACTCTTGAATGTGAAAGTCTTCCAT37960.12225785392376534No Hit
TGTTCACACTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCAC37810.12177474860004128No Hit
GGTTTCCATGGTTTCAAGACTAAAACATGAGAATCTCATTCAACTGGTTG35340.11381961426938532No Hit
CTAACACCGATATGCTTCGGGGAGCTGTAAGTGAGCTTTGATCCGGAGAT34900.11240250531979479No Hit
CAGAGCTGCTGTTGTTATTTCTATTTCTGGTAGCTCTGTAGAAGACAAAG33980.10943945933428731No Hit
TTTTTTTTTTTTTTTTTTAACCAAACTTAATTTTTATTTTTGTCAGAAAG32820.10570344483082136No Hit
TTTTTTTTTTTTTTTTGCCGATCGAGTAAAAAGTTAATAAATCTTAATGA32790.10560682376607652No Hit
TTTTTTTTTTTTTTTTTTTAACCAAACTTAATTTTTATTTTTGTCAGAAA31910.10277260586689548No Hit
CCGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAA31750.10225729352158983No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)