FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_4G_1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_4G_1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13618539
Sequences flagged as poor quality0
Sequence length101
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG2138631.5703813749771545No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG1873791.3759111752002178No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG1868761.3722176806190443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1256930.9229550981937196No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG1061210.779239241448734No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT1048080.7695979722935038No Hit
TTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT909490.6678322836245503No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT812010.596253386651828No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG782120.5743053641804015No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT719910.5286249868653311No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC712370.5230884164593574No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG491060.3605819978192962No Hit
TTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441210.3239774839283421No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA379810.27889188407067744No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340170.24978450331566404No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC322460.23678017150004121No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA300580.22071383721851517No Hit
TTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283880.2084511414917562No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC274810.20179110255512722No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC274060.20124038268715902No Hit
TTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC263960.19382402179852037No Hit
TTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCG224380.1647606986329444No Hit
TTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC209730.1540033038786319No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG208460.1530707515688724No Hit
TTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190500.13988284646392687No Hit
TTTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATC180210.13232696987540293No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA175010.12850864545748997No Hit
TTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTCT171000.12556412989675325No Hit
TTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC168740.12390462736127568No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT166700.12240666932040213No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA163730.120225818643248No Hit
TTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTC153560.112758057233599No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT142410.10457068852980483No Hit
TTTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGT140130.10289650013118147No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC139560.10247795303152563No Hit
TTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTA136310.10009150027033002No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)