FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_3R_1-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_3R_1-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5389275
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG2821105.234655867440425No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG2650884.918806332948309No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1903413.5318479758409063No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT1169432.1699208149519182No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG1002211.8596378919242385No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC809371.5018161069902722No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT579451.0751910043558734No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG543951.0093194353600439No Hit
TTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTA417570.7748166497348902No Hit
TTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAA408540.7580611492269369No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT403080.7479299163616627No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG329990.6123087057164461No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG272020.5047432168519885No Hit
TTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC241450.4480194460293825No Hit
TTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAAT222810.4134322334636848No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG220790.4096840484109644No Hit
TTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAAT209580.3888834769055207No Hit
TTTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAA163590.30354732315571203No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC163030.30250822234901725No Hit
TTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAATC158380.29387997457914095No Hit
TTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCG151380.28089121449545623No Hit
TTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC150070.27846046082265236No Hit
TTTTTTTTTTTTTTTTTGCTTATTGATATGCTTAAACTCAGCGGGTAATC144830.26873744613143696No Hit
TTTTTTTTTTTTTTTTTGCCATTGTAGCACGTGTGTGGCCCAGCCCATAA144000.26719735029294295No Hit
TTTTTTTTTTTTTTTTTTGCTTATTGATATGCTTAAACTCAGCGGGTAAT143000.2653418131381308No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC142210.26387593878582927No Hit
TTTTTTTTTTTTTTTTTTGCCATTGTAGCACGTGTGTGGCCCAGCCCATA141090.2617977371724397No Hit
TTTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGT132830.24647100027369173No Hit
TTTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTAT131070.24320525488122244No Hit
TTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC119550.221829466857787No Hit
TTTTTTTTTTTTTTTTTTGTCACTACCTCCCCGTGTCAGGATTGGGTAAT118180.21928738095569442No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96620.17928199989794544No Hit
TTTTTTTTTTTTTTTTTTGATATGCTTAAACTCAGCGGGTAATCCCGCCT96320.17872533875150182No Hit
TTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTCT95700.17757490571551832No Hit
TTTTTTTTTTTTTTTTTGTCACTACCTCCCCGTGTCAGGATTGGGTAATT85380.1584257622778574No Hit
TTTTTTTTTTTTTTTTTTGAATATTTGCTACTACCACCAAGATCTGCACC80530.1494264070770187No Hit
TTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA80050.1485357492427089No Hit
TTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGC77700.14417523692890044No Hit
TTTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGG76470.14189292622848157No Hit
TTTTTTTTTTTTTTTTGCCATTGTAGCACGTGTGTGGCCCAGCCCATAAG70960.13166891650546686No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC68630.12734551493475466No Hit
TTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGGG67050.12441376623015155No Hit
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTCCTTGTTCGCTATCG66890.12411688028538162No Hit
TTTTTTTTTTTTTTTTTGATATGCTTAAACTCAGCGGGTAATCCCGCCTG66170.12278089353391688No Hit
TTTTTTTTTTTTTTTTTTATGCTTAAACTCAGCGGGTAATCCCGCCTGAC65400.12135212992471157No Hit
TTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAA64990.12059135969123862No Hit
TTTTTTTTTTTTTTTTAATCTTTACCTCGCGGTACTTGTTCGCTATCGGT64860.12035013986111305No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTTTTCGCTATCGG63990.11873582253642652No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63580.11797505230295355No Hit
TTTTTTTTTTTTTTTTTGCTTAAACTCAGCGGGTAATCCCGCCTGACCTG62120.11526596805692788No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA61930.11491341599751358No Hit
TTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAATC60690.11261254992554658No Hit
TTTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATC58270.10812215001090128No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTCCTTGTTCGCTATCGG56240.10435540958663271No Hit
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTTTTCGCTATCG55650.10326064266529358No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA55200.10242565094562812No Hit
TTTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGT55130.10229576334479128No Hit
TTTTTTTTTTTTTTTTGCTTATTGATATGCTTAAACTCAGCGGGTAATCC55010.10207309888621383No Hit
TTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAA54380.1009041104786822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)