FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_3G_1-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_3G_1-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7989062
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG3175233.9744715962900274No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG2252842.8199055158165No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG2081322.605211976074288No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT975421.2209443361435923No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC974401.219667590513129No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG851761.0661577041209593No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT809491.0132478631408794No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG782280.9791887958811686No Hit
TTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTA599050.7498377156166769No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG581000.727244324803087No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT476860.5968910993555939No Hit
TTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTC469570.587766123231989No Hit
TTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAA412180.5159304058473949No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG353810.44286801128843406No Hit
TTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTCG306880.38412519517310045No Hit
TTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA296830.37154549557883015No Hit
TTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAA288680.3613440476491483No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC251110.31431725026041857No Hit
TTTTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAG249140.3118513787976611No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA238510.2985456865899902No Hit
TTTTTTTTTTTTTTTTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGG231200.2893956762383369No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC227420.28466420713720836No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC213080.266714665626578No Hit
TTTTTTTTTTTTTTTTTTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGT210340.263284976383961No Hit
TTTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGT209940.262784291822995No Hit
TTTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGG206590.2585910586249049No Hit
TTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC203830.2551363351542396No Hit
TTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAA193430.24211853656912413No Hit
TTTTTTTTTTTTTTTTTTGACTTTCCAGCACCGGGCAGGTGTCAGACTCT188210.23558460304851808No Hit
TTTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAA187940.23524664096986606No Hit
TTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAAA185140.23174184904310421No Hit
TTTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGG182800.2288128443614532No Hit
TTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAAT179810.2250702272682325No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA174120.21794798938849141No Hit
TTTTTTTTTTTTTTTTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTT173170.21675886355619722No Hit
TTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCG170960.21399258135686017No Hit
TTTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTAT163670.20486760523325517No Hit
TTTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTT162040.2028273156473188No Hit
TTTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATC155100.19414043851455903No Hit
TTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAAT153170.19172463550789817No Hit
TTTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACG152380.19073578349999035No Hit
TTTTTTTTTTTTTTTTTTCTCTGGCTACTAAGATGTTTCAGTTCGCCAGG151770.18997223954451725No Hit
TTTTTTTTTTTTTTTTTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTC142530.1784064261862031No Hit
TTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC138780.17371250842714703No Hit
TTTTTTTTTTTTTTTTTGACTTTCCAGCACCGGGCAGGTGTCAGACTCTA134480.16833014939676275No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA128590.16095756923653867No Hit
TTTTTTTTTTTTTTTTTTGTCACTACCTCCCCGTGTCAGGATTGGGTAAT121720.15235831190194793No Hit
TTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTCT119910.1500927142635769No Hit
TTTTTTTTTTTTTTTTTTGCCTTGCGACCATACTCCCCCCGGAACCCAAA118410.14821514715995446No Hit
TTTTTTTTTTTTTTTTTTGCTTATTGATATGCTTAAACTCAGCGGGTAAT118020.14772697971301263No Hit
TTTTTTTTTTTTTTTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGA117460.14702602132766024No Hit
TTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC115940.14512341999598952No Hit
TTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGGG115510.1445851840929511No Hit
TTTTTTTTTTTTTTTTTTCTTAAAATCCCAAATCCGCTCTCGCTCGCCGC111350.1393780646589049No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110240.1379886650022243No Hit
TTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACGG107950.13512224589069405No Hit
TTTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCC107810.13494700629435596No Hit
TTTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCA105200.13168003953405294No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA103910.13006533182493765No Hit
TTTTTTTTTTTTTTTTTGCCTTGCGACCATACTCCCCCCGGAACCCAAAA99390.12440759628602206No Hit
TTTTTTTTTTTTTTTTTTAAAATCCCAAATCCGCTCTCGCTCGCCGCTAC99300.12429494225980472No Hit
TTTTTTTTTTTTTTTTTTGAATATTTGCTACTACCACCAAGATCTGCACC98820.12369412078664554No Hit
TTTTTTTTTTTTTTTTTCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGT98460.12324350468177615No Hit
TTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAATC96180.12038960268427007No Hit
TTTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGT93980.11763583759895717No Hit
TTTTTTTTTTTTTTTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTTC92350.11559554801302081No Hit
TTTTTTTTTTTTTTTTTTCTCCGCTTATTGATATGCTTAAACTCAGCGGG92110.11529513727644121No Hit
TTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGT90780.11363036111122933No Hit
TTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCA88840.11120204099054432No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT88510.11078897622774739No Hit
GTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG87240.10919930274668041No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTCCTTGTTCGCTATCGG87010.10891140912412496No Hit
TTTTTTTTTTTTTTTTTGCTTATTGATATGCTTAAACTCAGCGGGTAATC84590.10588226753028077No Hit
TTTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCT84430.10568199370589437No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC80490.10075025078037947No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)