FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_2R_2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_2R_2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10971235
Sequences flagged as poor quality0
Sequence length101
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG1273461.1607262081251564No Hit
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG1244191.1340473520073173No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG935160.8523744136371155No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG911690.8309821091244514No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG809670.7379934893382559No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT549020.5004176831505295No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT496380.45243766996149476No Hit
TTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTA453380.41324427013002635No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG407610.37152608616987964No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG359650.3278117732415721No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC349190.3182777508639638No Hit
TTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAA312860.28516388537844645No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT307620.28038775944549543No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA199810.18212170279827203No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC175600.16005490721874063No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC163650.14916278796325116No Hit
TTTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAA157380.1434478433831743No Hit
TTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAAT157280.14335669594170575No Hit
TTTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGG154070.14043086307056588No Hit
TTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTC147340.13429664025973376No Hit
TTTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGT141630.1290921213518806No Hit
TTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAA138200.12596576410951002No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG135770.1237508812818247No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA135590.12358681588718134No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC128200.1168510199626569No Hit
TTTTTTTTTTTTTTTTTTGTTTCGTTTAGTACGAGATGGCTTCACACCTC124120.11313220435074084No Hit
TTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA121100.11037955161839119No Hit
TTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAAT115870.105612540429587No Hit
TTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC113320.10328828067213947No Hit
TTTTTTTTTTTTTTTTTCGTTTAGTACGAGATGGCTTCACACCTCGCGGT113120.10310598578920241No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)