FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_2G_2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_2G_2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9751671
Sequences flagged as poor quality0
Sequence length101
%GC28

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG986481.0116009861284287No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG918240.9416232356485366No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG750230.7693348145153789No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG504070.5169062820105396No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT453040.4645767889421208No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG447430.45882392874000777No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG353390.3623891741220556No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC300700.30835740869436634No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT298370.3059680745997276No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT287050.29435980766783454No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA285300.2925652434336638No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC277800.28487425385864634No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA221090.22672011801874778No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG215640.22113133226090176No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA142540.14616982053639832No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC119480.12252259125641134No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA118180.12118948639674165No Hit
TTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115230.11816436383056811No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT114370.11728246369263279No Hit
TTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC102860.10547935835817267No Hit
TTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTA102520.10513070016410521No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC102320.1049256071087714No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC101700.10428981863723663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)