FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_2G_18-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_2G_18-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8766315
Sequences flagged as poor quality0
Sequence length101
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1298711.4814776790475814No Hit
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG954541.0888725764474583No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG663570.7569543188899783No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG659770.7526195442440752No Hit
TTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTC438550.5002672160423165No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA435770.4970959861697874No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC397790.453771054314156No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA301910.344397845617001No Hit
TTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTCG275580.3143624202415724No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT275500.31427116182797443No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC252020.28748681743697324No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT215880.24626082909409483No Hit
TTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTA214330.24449269733063436No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG206970.23609692327962203No Hit
TTTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACG199310.22735893017761738No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG197620.22543109619036047No Hit
TTTTTTTTTTTTTTTTTTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGT188020.2144800865586053No Hit
TTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAA187690.21410364560251371No Hit
TTTTTTTTTTTTTTTTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTT182920.20866236269173535No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA179970.2052972086903106No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG166830.19030801425684568No Hit
TTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA161850.18462717801037268No Hit
TTTTTTTTTTTTTTTTTTCTTAAAATCCCAAATCCGCTCTCGCTCGCCGC147180.16789266641684678No Hit
TTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAA144780.16515491400890797No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA142940.16305597049615486No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT139960.15965659458963088No Hit
TTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACGG138490.15797972123976836No Hit
TTTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTT129620.14786144463209455No Hit
TTTTTTTTTTTTTTTTTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTC123910.14134787536154017No Hit
TTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCA121220.13827931120430878No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG119250.136032072769459No Hit
TTTTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAG116830.13327150575812072No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC116330.13270114067313346No Hit
TTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAAA113080.12899376762071635No Hit
TTTTTTTTTTTTTTTTTTAAAATCCCAAATCCGCTCTCGCTCGCCGCTAC112980.1288796946037189No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT112300.12810399808813625No Hit
TTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAA105910.1208147323019992No Hit
TTTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGG93010.10609931310932814No Hit
TTTTTTTTTTTTTTTTTTGTTGTTGTTGTTGCTTCTCTGCAAATTGCGTT91400.10426273753566921No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT88530.1009888419478424No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)