FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_2G_1-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_2G_1-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6008475
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG2294093.8180902808116866No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG1689082.811162566208564No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG1551702.582518858778642No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT735361.223871281814437No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC714161.1885877864183507No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG698801.1630238954143939No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT608111.0120870936468904No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG581290.9674501433392No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG546700.90988145910568No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT396810.6604171607604259No Hit
TTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTA347040.5775841623706515No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG295710.49215483130078763No Hit
TTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTC278730.4638947486675072No Hit
TTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAA247270.4115353729523714No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA212300.3533342487070346No Hit
TTTTTTTTTTTTTTTTTTGACTTTCCAGCACCGGGCAGGTGTCAGACTCT204530.34040251478120487No Hit
TTTTTTTTTTTTTTTTTTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGT203090.3380058999995839No Hit
TTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA190490.3170355206604005No Hit
TTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTCG185410.3085807962919043No Hit
TTTTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAG185130.3081147878621447No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC183270.30501916043588434No Hit
TTTTTTTTTTTTTTTTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTT179940.29947698875338585No Hit
TTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAA179580.2988778350579806No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC175130.29147162965644363No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC165330.27516133461485653No Hit
TTTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGG157900.26279546806802057No Hit
TTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC152820.2543407436995244No Hit
TTTTTTTTTTTTTTTTTGACTTTCCAGCACCGGGCAGGTGTCAGACTCTA150010.2496640162437224No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA146100.2431565413852933No Hit
TTTTTTTTTTTTTTTTTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTC140790.23431902437806598No Hit
TTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAA129440.2154290398145952No Hit
TTTTTTTTTTTTTTTTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGG124280.20684117018045342No Hit
TTTTTTTTTTTTTTTTTTGAATATTTGCTACTACCACCAAGATCTGCACC123270.2051602112016776No Hit
TTTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGG121510.2022310153574742No Hit
TTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCG121030.20143214376360058No Hit
TTTTTTTTTTTTTTTTTTCTCTGGCTACTAAGATGTTTCAGTTCGCCAGG120650.2007997037517839No Hit
TTTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACG117690.19587332892289638No Hit
TTTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATC117160.19499124153799424No Hit
TTTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTAT116630.19410915415309207No Hit
TTTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGT116060.19316049413536712No Hit
TTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAAA115250.19181239832070535No Hit
TTTTTTTTTTTTTTTTTTGTCACTACCTCCCCGTGTCAGGATTGGGTAAT106870.1778654317443278No Hit
TTTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAA105900.17625104539837477No Hit
TTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAAT105310.17526909906423843No Hit
TTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC101360.16869505157298648No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98000.16310295041587092No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA97850.1628533030427854No Hit
TTTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTT97750.16268687146072838No Hit
TTTTTTTTTTTTTTTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTTC96780.1610724851147754No Hit
TTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAAT95810.15945809876882236No Hit
TTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC93360.15538052500842559No Hit
TTTTTTTTTTTTTTTTTTCTTAAAATCCCAAATCCGCTCTCGCTCGCCGC93020.15481465762943175No Hit
TTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTCT91700.15261776074627922No Hit
TTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACGG87780.14609364272964437No Hit
TTTTTTTTTTTTTTTTTCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGT84030.13985245840250646No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA82860.13790520889243943No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT81140.13504258568105884No Hit
TTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGGG80320.13367784670819136No Hit
TTTTTTTTTTTTTTTTTTAAAATCCCAAATCCGCTCTCGCTCGCCGCTAC79670.13259604142482076No Hit
TTTTTTTTTTTTTTTTTTTGGCTACTAAGATGTTTCAGTTCGCCAGGTTG75180.12512326339046098No Hit
TTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCA74300.12365866546835927No Hit
TTTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGT73990.12314272756398253No Hit
TTTTTTTTTTTTTTTTTTGCCTTGCGACCATACTCCCCCCGGAACCCAAA73940.12305951177295404No Hit
TTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGT72340.12039660646004187No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC69360.11543694531474293No Hit
TTTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCC68590.11415542213290394No Hit
TTTTTTTTTTTTTTTTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTAC67940.11307361684953336No Hit
TTTTTTTTTTTTTTTTTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTT67870.11295711474209347No Hit
TTTTTTTTTTTTTTTTTTCTCCGCTTATTGATATGCTTAAACTCAGCGGG67800.11284061263465354No Hit
TTTTTTTTTTTTTTTTTTGCTTATTGATATGCTTAAACTCAGCGGGTAAT66890.11132608523793475No Hit
TTTTTTTTTTTTTTTTTTTGACTTTCCAGCACCGGGCAGGTGTCAGACTC65660.10927897677863352No Hit
TTTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCA65450.10892947045631378No Hit
TTTTTTTTTTTTTTTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGA64840.10791423780576602No Hit
TTTTTTTTTTTTTTTTTGCCTTGCGACCATACTCCCCCCGGAACCCAAAA64320.10704879357906956No Hit
TTTTTTTTTTTTTTTTTGAATATTTGCTACTACCACCAAGATCTGCACCG63570.10580055671364197No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG62760.10445246089898019No Hit
TTTTTTTTTTTTTTTTTGTCACTACCTCCCCGTGTCAGGATTGGGTAATT60630.10090746820116585No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)