FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_1R_2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_1R_2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7690793
Sequences flagged as poor quality0
Sequence length101
%GC28

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG598160.7777611489478393No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG597460.7768509697244484No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG425850.5537140318300077No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG422350.5491631357130533No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA421830.5484870025756772No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC387410.5037321899055143No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA295390.38408262971061635No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG253610.3297579326345151No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT247300.32155331706366297No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT224210.2915304052520982No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA196630.2556693438504976No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG175500.22819493386442724No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC148490.19307501840187352No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG146770.19083857802439877No Hit
TTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCA142140.18481839258968483No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT142110.18477938490868237No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT141780.18435030041765524No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA129900.16890325874067863No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT112860.14674689593127782No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG112580.14638282424192148No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC110570.14376930961475626No Hit
TTTTTTTTTTTTTTTTTTGTAAAAACCCATCTCATAAATAACGTCATGCA98460.12802320905009407No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC89830.11680199948171796No Hit
TTTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACG88050.11448754374223828No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC86630.11264118017478822No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)