FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_1R_18-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_1R_18-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4532833
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG1516923.3465164059651No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG1085342.394396616861905No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1050042.316520374785482No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG943402.0812591154361963No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT419540.9255580340153718No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC417480.9210134147893823No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT361160.7967644076011625No Hit
TTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTA298350.6581976437252377No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG294760.6502776519673238No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG262450.5789977261460989No Hit
TTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAA206200.45490314776652924No Hit
TTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTC189660.41841382640834107No Hit
TTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAA181680.4008089422222262No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG176300.38893998521454465No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC175170.38644706301776394No Hit
TTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA169730.3744457384598109No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA165880.3659521539840537No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT145080.3200647365565861No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA135260.2984005808288106No Hit
TTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTCG119670.26400707901658854No Hit
TTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAAA114430.25244697962620727No Hit
TTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAA113950.25138803922403496No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG100450.22160534041293822No Hit
TTTTTTTTTTTTTTTTTTGCCTTGCGACCATACTCCCCCCGGAACCCAAA94090.20757438008415485No Hit
TTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAAT92330.20369159860952302No Hit
TTTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAA91620.2021252492646431No Hit
TTTTTTTTTTTTTTTTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTT90980.20071332872841335No Hit
TTTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGT89810.1981321614981183No Hit
TTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC80260.17706365974656468No Hit
TTTTTTTTTTTTTTTTTGCCTTGCGACCATACTCCCCCCGGAACCCAAAA79350.17505608523411298No Hit
TTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAAT77820.17168071270218868No Hit
TTTTTTTTTTTTTTTTTTGAATATTTGCTACTACCACCAAGATCTGCACC74790.16499615141347584No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC72580.16012061331180744No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC71030.15670111826312597No Hit
TTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCA70830.15625989309555416No Hit
TTTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGG66000.14560430529869509No Hit
TTTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACG64020.14123617613973424No Hit
TTTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTAT60970.13450749233426426No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT57380.12658750057635038No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA56360.12433725222173418No Hit
TTTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCA55060.12146928863251746No Hit
TTTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCC54190.1195499591535801No Hit
TTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC54110.1193734690865514No Hit
TTTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTT54110.1193734690865514No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG53010.11694673066490646No Hit
TTTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATC50730.11191676375458791No Hit
TTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCG50210.11076957831890122No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA48740.10752657333724847No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT48610.1072397769783268No Hit
TTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAATC48580.10717359320319103No Hit
TTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACGG47470.10472479352316752No Hit
TTTTTTTTTTTTTTTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTTC46880.1034231792788307No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)