FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_1R_1-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_1R_1-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4768755
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG3031676.357361617445225No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG2097744.398925925110432No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG1679963.522848206712234No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC812831.7044910044655261No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT703341.4748922936909108No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT667251.3992121633424237No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG546071.145099716802394No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG527891.1069765588712357No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG492371.0324917090519434No Hit
TTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTA354630.7436532176637298No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT284300.596172376228177No Hit
TTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAA246020.5158998522675206No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC242760.5090636864338806No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA223460.46859190711202403No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC212460.44552508988195033No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG202310.42424070852874596No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA179140.3756536035086726No Hit
TTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA175330.3676640968135289No Hit
TTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAA160070.33566413036526305No Hit
TTTTTTTTTTTTTTTTTTGTCACTACCTCCCCGTGTCAGGATTGGGTAAT157130.32949899921467973No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC156170.32748589516550963No Hit
TTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTC151930.31859468561500853No Hit
TTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCG138790.29104032394199325No Hit
TTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC127110.2665475580104241No Hit
TTTTTTTTTTTTTTTTTTGAATATTTGCTACTACCACCAAGATCTGCACC124920.2619551643982549No Hit
TTTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGG118440.24836671206635696No Hit
TTTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTAT115620.24245321892191984No Hit
TTTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAA112950.23685427328516562No Hit
TTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAA111500.233813647377565No Hit
TTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAAT109380.22936804260231444No Hit
TTTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGT105560.2213575660733252No Hit
TTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC100370.21047422230749951No Hit
TTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCA98990.20758038523681757No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96240.20181368092929913No Hit
TTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAAA93240.1955227307756427No Hit
TTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTCG91460.19179010035113986No Hit
TTTTTTTTTTTTTTTTTTGCTTATTGATATGCTTAAACTCAGCGGGTAAT88800.18621212454823113No Hit
TTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAAT88640.1858766072067028No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA85620.1795437173853553No Hit
TTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTCT85060.1783694066900061No Hit
TTTTTTTTTTTTTTTTTGTCACTACCTCCCCGTGTCAGGATTGGGTAATT82950.17394477174860107No Hit
TTTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATC78960.16557780804423797No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA77990.16354373416122236No Hit
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTTTTCGCTATCG77330.16215972512741794No Hit
TTTTTTTTTTTTTTTTTTCTCCGCTTATTGATATGCTTAAACTCAGCGGG75760.15886746121367107No Hit
TTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGGG72480.15198935571234No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT72080.15115056235851915No Hit
TTTTTTTTTTTTTTTTTTGCCTTGCGACCATACTCCCCCCGGAACCCAAA71030.1489487298047394No Hit
TTTTTTTTTTTTTTTTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGG70040.14687271625403275No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG69320.1453628882171552No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTTTTCGCTATCGG66040.13848478271582415No Hit
TTTTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAG64270.13477312212516684No Hit
TTTTTTTTTTTTTTTTTTGTAAAAACCCATCTCATAAATAACGTCATGCA64210.1346473031220937No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT63850.13389238910365495No Hit
TTTTTTTTTTTTTTTTTGCTTATTGATATGCTTAAACTCAGCGGGTAATC63690.13355687176212658No Hit
TTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC62740.13156473754680204No Hit
TTTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCT61750.1294887239960954No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTTTTCGCTATCGG61680.12934193515917677No Hit
TTTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGT59010.12374298952242252No Hit
TTTTTTTTTTTTTTTTTGCCTTGCGACCATACTCCCCCCGGAACCCAAAA58510.12269449783014644No Hit
TTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAATC56160.11776658687644888No Hit
TTTTTTTTTTTTTTTTTGAATATTTGCTACTACCACCAAGATCTGCACCG55650.11669712535032728No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC55520.11642451751033552No Hit
TTTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCC55380.11613093983649819No Hit
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTCCTTGTTCGCTATCG52290.10965126117823205No Hit
TTTTTTTTTTTTTTTTTTGATATGCTTAAACTCAGCGGGTAATCCCGCCT51490.10797367447059032No Hit
TTTTTTTTTTTTTTTTTTTGTCACTACCTCCCCGTGTCAGGATTGGGTAA50860.10665257493832248No Hit
TTTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGG50790.10650578610140382No Hit
TTTTTTTTTTTTTTTTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTAC50750.10642190676602174No Hit
TTTTTTTTTTTTTTTTTTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGT50670.10625414809525757No Hit
TTTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCA50620.10614929892602996No Hit
TTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCGC50030.10491207872914418No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTCCTTGTTCGCTATCGG48860.10245860816921817No Hit
TTTTTTTTTTTTTTTTTTCTTCTTTACCTCGCGGTACTTGTTCGCTATCG47770.10017289628005634No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)