FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_14R_1-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_14R_1-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5747905
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG1741243.029347214332874No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG1209472.1041927450088336No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG1192062.073903448299859No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG555900.9671349822239581No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT516020.8977531813765189No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG505750.8798858018704206No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC472700.8223865912884782No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT397840.6921478347328288No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG380950.6627632154672006No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT288650.5021829692731526No Hit
TTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA288430.5018002211240443No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC279300.4859161729360523No Hit
TTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAA273560.47592992577295556No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA272570.4742075591019685No Hit
TTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTC265030.46108973617344057No Hit
TTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTA215160.374327689827859No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA208130.36209714669953663No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG203410.3538854591368507No Hit
TTTTTTTTTTTTTTTTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGG189790.33018986917842236No Hit
TTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAA187960.3270061004835675No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC175350.3050676724824088No Hit
TTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAAA165880.2885921044276132No Hit
TTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTCG161420.28083275558660065No Hit
TTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAA145300.25278775484285143No Hit
TTTTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAG143470.24960398614799653No Hit
TTTTTTTTTTTTTTTTTTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGT135720.2361208127135017No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC129340.22502111638936273No Hit
TTTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACG120980.2104766867232496No Hit
TTTTTTTTTTTTTTTTTTGCCTTGCGACCATACTCCCCCCGGAACCCAAA119370.2076756661775029No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA119050.20711894159698185No Hit
TTTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGG115680.2012559358583693No Hit
TTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCG115020.20010769141104454No Hit
TTTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGG111790.19448825267640993No Hit
TTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCA109260.19008664896166516No Hit
TTTTTTTTTTTTTTTTTGCCTTGCGACCATACTCCCCCCGGAACCCAAAA97620.16983579234521098No Hit
TTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC97390.16943564655296148No Hit
TTTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCC95600.16632146843067172No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA94970.16522541691277082No Hit
TTTTTTTTTTTTTTTTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTT94490.1643903300419892No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT93610.16285933744555625No Hit
TTTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCA90120.15678755998924826No Hit
TTTTTTTTTTTTTTTTTTGTCACTACCTCCCCGTGTCAGGATTGGGTAAT89290.1553435556085217No Hit
TTTTTTTTTTTTTTTTTTGATAAGGTTTAGTGGACTTCTCGCGACGTCGC89190.15516957917710889No Hit
TTTTTTTTTTTTTTTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGA88660.15424750409062085No Hit
TTTTTTTTTTTTTTTTTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTC87000.15135949532916776No Hit
TTTTTTTTTTTTTTTTTTAAAATCCCAAATCCGCTCTCGCTCGCCGCTAC84800.14753201383808534No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG84590.14716666333211842No Hit
TTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACGG84270.14660993875159734No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81950.14257368554281952No Hit
TTTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTT81210.14128625995036453No Hit
TTTTTTTTTTTTTTTTTTGAATATTTGCTACTACCACCAAGATCTGCACC77070.1340836356898731No Hit
TTTTTTTTTTTTTTTTTTCTTAAAATCCCAAATCCGCTCTCGCTCGCCGC77040.13403144276044923No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC76430.13297018652883094No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT75630.13157837507752826No Hit
TTTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTAT70990.12350586865997264No Hit
TTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGGG69830.12148774205558374No Hit
TTTTTTTTTTTTTTTTTTCTCTGGCTACTAAGATGTTTCAGTTCGCCAGG69540.12098321040448651No Hit
TTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAAA68850.11978277302773795No Hit
TTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTCT68680.11948701309433613No Hit
TTTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATC67120.11677298076429585No Hit
TTTTTTTTTTTTTTTTTTGTAAAAACCCATCTCATAAATAACGTCATGCA66970.1165120161171766No Hit
TTTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGT66300.11534637402671061No Hit
TTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC66110.11501581880702622No Hit
TTTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAA64140.11158848310819332No Hit
TTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC62760.1091876083546962No Hit
TTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAAT60990.106108225518689No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)