FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_14G_2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_14G_2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3156742
Sequences flagged as poor quality0
Sequence length101
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG598531.8960371167488506No Hit
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG576131.8250778809291353No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG473351.4994890301456376No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG398531.2624725112156776No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT250440.7933495990486393No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT242370.7677852672153759No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240540.7619881510747473No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG174800.5537354652359933No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG165850.5253834491383838No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC153000.4846769232328774No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC148030.46893284278537806No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA145720.46161517159147No Hit
TTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTA142580.45166820728459917No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT127820.40491113939625095No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA111570.3534340151966806No Hit
TTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAA95960.30398429773481644No Hit
TTTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGG81990.25972981003832435No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC78580.24892753351398372No Hit
TTTTTTTTTTTTTTTTTTGACTTTCCAGCACCGGGCAGGTGTCAGACTCT76270.24160986232007556No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA73150.23172625447375805No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC72800.23061751641407502No Hit
TTTTTTTTTTTTTTTTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTT67330.21328952445274274No Hit
TTTTTTTTTTTTTTTTTTGATAAGGTTTAGTGGACTTCTCGCGACGTCGC66510.21069190957005673No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA64920.205655070956068No Hit
TTTTTTTTTTTTTTTTTGACTTTCCAGCACCGGGCAGGTGTCAGACTCTA53320.16890832383514395No Hit
TTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC53060.16808468984795083No Hit
TTTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAA51550.16330127707617537No Hit
TTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCA51140.16200246963483236No Hit
TTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGGG49820.15782094323831342No Hit
TTTTTTTTTTTTTTTTTCGCTTATTGATATGCTTAAACTCAGCGGGTAAT49530.15690227456029032No Hit
TTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTCG47710.15113683664993846No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT47270.14974299451776546No Hit
TTTTTTTTTTTTTTTTTTGTTTCGTTTAGTACGAGATGGCTTCACACCTC45190.14315392262022048No Hit
TTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGTC44530.14106315942196102No Hit
TTTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACG44430.1407463771191944No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG44200.14001777782283126No Hit
TTTTTTTTTTTTTTTTTTGTAAAAACCCATCTCATAAATAACGTCATGCA43610.1381487622365084No Hit
TTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTCT41490.13143297741785678No Hit
TTTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGT40840.12937389244987396No Hit
TTTTTTTTTTTTTTTTTTGTTTAGTACGAGATGGCTTCACACCTCGCGGT40710.1289620754562774No Hit
TTTTTTTTTTTTTTTTTCGTTTAGTACGAGATGGCTTCACACCTCGCGGT40700.12893039722600075No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT39260.12436873206616188No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37090.11749455609612695No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC36680.11619574865478395No Hit
TTTTTTTTTTTTTTTTTTCGTTTAGTACGAGATGGCTTCACACCTCGCGG35900.11372484669320458No Hit
TTTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGT35080.11112723181051856No Hit
TTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAAT34960.11074709304719867No Hit
TTTTTTTTTTTTTTTTTTGAATAACGTCATGCATTACATGTTAATTATTA34740.11005017198111218No Hit
TTTTTTTTTTTTTTTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTTC32050.101528728036691No Hit
TTTTTTTTTTTTTTTTTTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGT31900.1010535545825411No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)