FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_13R_2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_13R_2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7630883
Sequences flagged as poor quality0
Sequence length101
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG835531.094932264064329No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG785761.029710454216111No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC640060.8387758009132101No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA605160.793040595695151No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG592100.7759259315075332No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG498950.6538561788983005No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA481900.6315127620224291No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG400790.5252209999812604No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT365220.478607783660161No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA282990.370848301566149No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG277450.363588329161907No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT276100.36181920231249776No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC245530.3217583076558768No Hit
TTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTA222050.29098860511948615No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT221470.2902285358064067No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA220300.2886952925369187No Hit
TTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCA216790.2840955627284549No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG206360.2704274197363529No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG205850.26975908292657613No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT205070.26873692074691746No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC189410.24821504929377114No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT182350.2389631711035276No Hit
TTTTTTTTTTTTTTTTTTGTAAAAACCCATCTCATAAATAACGTCATGCA162330.21272767515895605No Hit
TTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAA149020.19528539488811453No Hit
TTTTTTTTTTTTTTTTTTGACTTTCCAGCACCGGGCAGGTGTCAGACTCT138330.1812765311694597No Hit
TTTTTTTTTTTTTTTTTTGTTTCGTTTAGTACGAGATGGCTTCACACCTC117640.15416302412184804No Hit
TTTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACG110590.14492425057493347No Hit
TTTTTTTTTTTTTTTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGG107080.14032452076646962No Hit
TTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCAT105500.13825398712049444No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC102950.13491230307161045No Hit
TTTTTTTTTTTTTTTTTCGTTTAGTACGAGATGGCTTCACACCTCGCGGT99030.12977528288665938No Hit
TTTTTTTTTTTTTTTTTTGTTTAGTACGAGATGGCTTCACACCTCGCGGT98860.12955250395006712No Hit
TTTTTTTTTTTTTTTTTTAGCTACTTAGATGTTTCAGTTCGCTAAGTTTT95780.12551627380474842No Hit
TTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCAT95320.12491346021161641No Hit
TTTTTTTTTTTTTTTTTGACTTTCCAGCACCGGGCAGGTGTCAGACTCTA95110.1246382627017083No Hit
TTTTTTTTTTTTTTTTTTGAATAACGTCATGCATTACATGTTAATTATTA93910.12306570550223347No Hit
TTTTTTTTTTTTTTTTTTCATAAATAACGTCATGCATTACATGTTAATTA90170.11816456889720364No Hit
TTTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGC88740.1162906049011628No Hit
TTTTTTTTTTTTTTTTTTCGTTTAGTACGAGATGGCTTCACACCTCGCGG84950.11132394507948817No Hit
TTTTTTTTTTTTTTTTTTACCTCGCGGTACTTGTTCGCTATCGGTCTCTC84690.1109832243529353No Hit
TTTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTC84420.11062939898305348No Hit
TTTTTTTTTTTTTTTTTCCCAAATCCGCTCTCGCTCGCCGCTACTAACGG76720.10053882361975672No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)