FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n02_11R_2-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n02_11R_2-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5414296
Sequences flagged as poor quality0
Sequence length101
%GC29

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG451230.833404749204698No Hit
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG439100.811001097834326No Hit
TTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGG328120.606025233936231No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG308580.569935592734494No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA287490.5309831601375322No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC252750.46681969364068754No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG246770.45577485974169124No Hit
TTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGGT193600.3575718800745286No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA186520.34449538776601796No Hit
TTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCGGT173230.3199492602547035No Hit
TTTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCG143740.2654823452578137No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA143460.2649651958444828No Hit
TTTTTTTTTTTTTTTTTTTGTCTTTACCTCGCGGTACTTGTTCGCTATCG121640.22466448084847965No Hit
TTTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATC115380.21310249753615243No Hit
TTTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTA109140.2015774534676346No Hit
TTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCA108800.2009494863228756No Hit
TTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGGT102180.18872259662198004No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA95600.17656958540870318No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT94960.17538752960680393No Hit
TTTTTTTTTTTTTTTTTTGTAAAAACCCATCTCATAAATAACGTCATGCA92850.17149043938491726No Hit
TTTTTTTTTTTTTTTTTTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGT82400.15218968449453077No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC79240.14635328397265313No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG77950.14397070274694992No Hit
TTTTTTTTTTTTTTTTTCCGCTTATTGATATGCTTAAACTCAGCGGGTAA74730.13802348449364424No Hit
TTTTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAG70640.1304694091346317No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT69980.1292504140889231No Hit
TTTTTTTTTTTTTTTTTTCTCTGGCTACTAAGATGTTTCAGTTCGCCAGG65300.12060663103753469No Hit
TTTTTTTTTTTTTTTTTTCTTTACCTCGCGGTACTTGTTCGCTATCGGTC58650.10832433247092513No Hit
TTTTTTTTTTTTTTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGG55780.10302355098428309No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)