FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n01_9G_1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n01_9G_1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12958051
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCGGTATATCAAAGCGAACTTCGAAAGGGGATCCGGTTAAAATTCCGG188540.14550027623752984No Hit
TGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTG166770.1286999101948279No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA164020.12657767746090828No Hit
GGGAGCTTCGGCGCAAGTGAAATACCACTACTTTTAACGTTATTTTACTT150180.11589705890183638No Hit
GAACGATATAGAGGAGAAAGGATTTGATGGATGAGGATGATTTGATTGAA139870.10794061545212318No Hit
GTCAGGCTGCAATTGATTATCTTCAGATGGCTCTTCATAATCCCAAAGAA136650.10545567385095182No Hit
CGTAGGTATAAGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCA136460.10530904686206283No Hit
GCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGT135250.10437526445913818No Hit
CTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCAAGGTGGTCCTTGC132680.10239194150416601No Hit
AATTTCTTCAACAGTTACAGCTTGACCCGCAGCTGCAGTTTGATCCACAG132510.10226074893516007No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)