FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n01_4G_1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n01_4G_1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3104913
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAGCTATATATGGGAACAGAGCGATGAAGGAAGAAGTGAAACACAGAT166620.5366333935926707No Hit
CGAAAATGGATGGCGCTTAAGCGCGCGACCTATACCCGGCCGTCGGGGCA165050.531576891204359No Hit
CACGAGTATACTGCGCTGTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC161230.5192738089601866No Hit
AGCATGTATAACGTCATAGGATTTCGATCCTATTGTGTTGGCCTTCGGGA157610.5076148671476463No Hit
CTGCAAATGAGATCGAGATCATTCTTATCCAAAAAGTCAGCGACTACATC154720.4983070379105631No Hit
CTTCTCTATAATCGTTACAAAAAAATCAAAGCATCACTAATTCACTATAT152700.4918012195510792No Hit
GGAAATTATAGCGTTTAAGGACTTGGTTGAAAGGCTTCTCTTGGAATTTA152340.4906417667741415No Hit
GTACACTATAGTTTCTTAAGATTGAATCCTGTTGCCGGTCTTGCGATGAT150370.48429698352256567No Hit
CTGCGGTTGGTCCTAGGATGCAAATGTATCCACCAGGTGGTCCACCAATG144080.4640387669477374No Hit
GGGGGGTATACCTGGACGATCAGATACCGTCCTAGTCTCAACCATAAACG131000.42191198271900054No Hit
AGTGGGTATAATTGTGCGGTGAAGACGGTTAAAGCGGAAGGAGCCATGGC130960.4217831546326741No Hit
GTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCA129630.4174996207623209No Hit
GCCAACATTGCCAATTTATCAGCAAACTTTGGTTTACCGTGAACCGTGAA127100.4093512443021753No Hit
CACGGGTATAAAAGCATGCAACGAGTATGTATAAGGAATAACGCCTGGTA121960.3927968352092313No Hit
TAAGCGTATATATGCCATCTCTGCAAATTTGATTCTTTAATTTTTGTGAC118690.3822651391520471No Hit
CGCCGACGTCTCCGGACTCCCTAACGTTGCCGTCAACCGCCACGTCCCGG117490.3784002965622547No Hit
AGAGTCCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCA102670.33066949057831896No Hit
CCGACACTGACACTGAGAGACGAAAGCTAGGGGAGCGAATGGGATTAGAT102430.32989652206036046No Hit
GTGGGCGAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATT98810.3182375802478201No Hit
CATTGCTATACATCAGACAGACTTTTGGGGTGCTAAGATCCAAAGTCGAG97710.3146948078738438No Hit
CGGAGCAATCCGGTCCGCCGATCGATTCGGGGCGTGGACCGACGCGGATT95430.3073516069532383No Hit
CCAGCATTAACCGCTTCAAGATCCTTCTGCAGTAAAGCCTTGTAGTATTG95030.30606332608997416No Hit
GCGCGTTATAAGGTGGGGAGTTTGGCTGGGGCGGCACATCTGTTAAAAGA92400.2975928794140126No Hit
CTGGGTTCAGAACGTCGTGAGACAGTTCGGTCCATATCCGGTGTGGGCGT91900.29598252833493244No Hit
TGTAATTATATGTGGGTTAAAGCGGCTAGCGATTGGTATTTGGCAAATTA89660.2887681555006533No Hit
GTGGGCTAGTTGCAGCCTACAATCCGAACTGAGAATGGTTTTATGGGATT88260.2842591724792289No Hit
ATGCTGTATATGTCGTACTCGATTCTATTGTCATGGGAAGCAGAGGACTC86140.277431283903929No Hit
CGCTGATTCTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTA85270.27462927302632956No Hit
GAAGATTATAATAAGGAGAGGGTTCGGATAATGTCGTGGGCTGAGCAAAT83440.26873538807689623No Hit
GGACATTGTCAGGTGGGGAGTTTGGCTGGGGCGGCACATCTGTTAAAAGA81080.26113453098363787No Hit
TATGGATATATGTGATGATGGCGACTATTTATATTGATTTATTCATTTTT79890.2573018954154271No Hit
AAAATATATAAAGGGTTTGTTTTTCAGCCGACCAAGTGTGACGTTGACTT78210.2518911157897178No Hit
GCCGCGTACAACACGACTATGGGCCGAACTCTGCCGAGGGAAGGAAATGG77930.2509893191854329No Hit
CGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGCTAGGTACCGTCAAG77600.24992648747324No Hit
TTGTATTATAGTTTCCTTCTTCTATAATGGGATGTGGTCAGACTAAACGC76980.24792965213518062No Hit
TACTTTTATAGCTATCGTTGGCTTTATAATGATAGATTCTGGTTTCGGTG76630.2468024063798245No Hit
AGAAGGTATAAGGGCAAGCGATATTAGGGAATTGCTGATTTTTTTCTAAT76590.24667357829349806No Hit
CCTGATTATAATCTTCTGTTGAATTACGTTAAGCATGTAATAATTAACAT76340.245868402753958No Hit
TGAATTTATAGGTTAGCGAGTGGATTAAGAAGATCTCTGCCAGGCCTGCT76270.2456429536028868No Hit
TTAAGTTATTAAGGGCGCACGGTGGATGCCTTGGCACTAGAAGCCGATGA75130.24197135314258403No Hit
GGAGTATATATGTCGTTACCGGTGATCAGTTTTATGTTTTGTTTTTTCTC75070.24177811101309443No Hit
ACACGGTATAGAGGAAAATGATAAGGTTGGAGCACAAAGTGAAGACATTA75060.2417459039915128No Hit
CCTCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA74430.2397168616318718No Hit
CTTTCAAGTGATCAATCATGGAGTTCCTTTGGAAGTTCTTGATGACGTCA74210.23900830715707652No Hit
GATAGCCGTAGTAACTGGTTGTGCATGTATATACTCATGTTTCTATCGTG73940.23813871757437324No Hit
GCAAAATATCCGTACACCTTGGTGGGATTCGAGCGTCATCTATGGAAGCA72820.2345315311572337No Hit
GTATCATATATGGGGATGCTAATGAATTTGGTTGCTTTAATTCTGAAGTT71010.22870206025096354No Hit
AGCAGGTATATTTCGAGAGATTGGTGAAGATTAAGACGAGAGTTGATAGT68820.22164872252459247No Hit
CAGGGATATAACTTTGGAGGTCGATGAGATTGCCACATGAAGAAACAAAC68770.22148768741668445No Hit
GCGGAAAAAGGTTTTGGATTCATCGAAGTAGAAGGACAAGACGACGTATT68650.22110120315770523No Hit
ACTTAATATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGAC68400.22029602761816516No Hit
CGTGGATATAAGTTGGTGGAGGAAGGTGTTGATTTAAAGATTCCCTCTCA67570.2176228448268921No Hit
GTGGGGTATAGCCGGATGTTGTGGATATTGAATGCGGTGGTGGTGATGAT67410.21710753248158646No Hit
GTGGCAACTAAACACGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACAC66970.2156904235319959No Hit
GTAGGAAATTTGAGAGGAGCTGTCCTTAGTACGAGAGGACCGGGATGGAC66910.2154971814025063No Hit
TCCATGTATATTTCTTGTTATATATGTAGTTATATATCCAGGTATAATTT66820.21520731820827188No Hit
ACTTTGTATAAGAATTGAACGGGTTTTGTGGATCGTCGGAAAACAAACCC66590.214466556711895No Hit
CACGAATATAGGTATTTATCTTCTGTTGTCGATTGCTGACATACCTTCTC65240.21011860879837857No Hit
GTCTGTTATAGCTGCATCTTCTTGAATCTGTGTGGTATGACCAAGGCTCT64660.2082506015466456No Hit
GCTCTCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCAGAGTGCT64300.20709114876970788No Hit
TTAGTCTATAGAGGAAACGGTGAGGATGGTGGAGGAATATAGCGCAGCCA64250.20693011366179986No Hit
CATCCATATACATCAGGACGAGCTGTAAGAATTCGAGCTCGGTACCGGAT63590.20480445023741406No Hit
AGCTGATATAAGGGGAGGTGGTGACAATAAATAACAATACCGGGCTCTTT62010.19971574082752078No Hit
GGAGTATATATGTCGGTACCGGTGATCAGTTTTATGTTTTGTTTTTTCTC61930.19945808465486795No Hit
TCCCCTTATATGATTTTTAAATGGATAAAACCCTAGTTAGTTATTCAAGA61160.1969781439930845No Hit
CGCTACTACTCGGAGCTCCATGACGCCGATCTGACGGCTGCAAATCTAAC60780.1957542771729836No Hit
GGCGAGCAGCATTATCAGGAGCTTGATTTGAGAGATTAAAATCTGCAACT59900.1929200592738025No Hit
CTAACATATAGAGTCGAGGTAGCGACAATTGGATTTTGTATCGCGTCTCC59700.19227591884217043No Hit
CGGAGACGGAGGACGAGATGAAGCGTCGATTCCTTCTGGATGTTGTAGTC59350.19114867308681435No Hit
ATACGCTATATTCCACTAATTAAGAAACATATCTTTATATTAATATATGT59220.1907299818062535No Hit
CCGGAATATAGGCCTCTTCACAGTGGCTCTTGAGAACCTTCCTAGATTAA58540.1885399043387045No Hit
TAGGTTTATACAGATCGTGGCCTTTTTTTGGATAAAAGACGTTGTTAATA56720.1826782264108527No Hit
GAGCCGTATATGTTTCTGTACAATATTGTGTGATCACTACCAAATGATCT56350.18148656661233342No Hit
GCTAGATATAAAGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAA56290.1812933244828438No Hit
CCTGTCTATAACTGTTGGAATGTGATTTTTCGATAGTTTGTTATTTTATT56190.18097125426702776No Hit
CGTGAAAGCAGAAGGAGTCTGCGGTGGGTCCATATTCGGTGCGACTTTCT54910.17684875550458257No Hit
ATTTCATATAATCTGTGGGCTGAGCAAATGTACAGAGAGAAGGAGCAGAC54630.17594695890029768No Hit
CGCGAATATATAGGCTCAACTCTGAACAGCTTCGGATCTTTTTCTGTATG54460.17539943953341045No Hit
CCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCAC52730.1698276247997931No Hit
GCCTTGTATATGCAGGACAGAGTTTCTTTGATAAGGAAGAAGAATGACAG52510.16911907032499784No Hit
GGCTAGTATATGGTTTTGGTAAGTGCCGGATTGGCCAACTCTTTAATGTG52260.16831389478545777No Hit
GCAGTATATAGAGATGCCTCCTTTCAGTTACCTTTTCGATGGTATTGAGA51830.1669289928574488No Hit
TTCGGGTATAGGATGCGATGATTATCATATAATTTCTGTTGAATTACGTT49050.15797544085776316No Hit
GCATGACGGTGGCCAGATTCGATGGAAGAGATGATCGACCCAAGCTTTTT48620.15659053892975425No Hit
CAACGGTTTACCGAATTCAGTCAAACTCCGAATGCCAAAGACTTATCCAT48090.15488356678592927No Hit
TCTGTTTATAGGGACTTTGGCTTGGGATGCTTGCGGCGCAGATCACGTGT48060.15478694572118445No Hit
CGTAAATATAGTGTGGATCGAATAATAAACGAGAACGTACGTTGTCGTTG47590.15327321570684913No Hit
CGGAAGTATAAACAGTTAAAGTAGTTTTAAAAACGAGTTATATATGTGAT46570.1499880995055256No Hit
ACACTATATACAGAAAAAAGCAAACCCAAAAAAGAGAACAGGCGAAAAAC46500.14976265035445438No Hit
CCTAGCTCTGTCTTCCGACTTCTCCATCCCGCTCACCAAAGAGTTTGACT44910.1446417339229795No Hit
TAAAGGTATATGGTTTTGTTGATCGTTCTTATTCTTCTTCAATTGAGTTT44400.14299917582231772No Hit
CGAATACAGCCTCAAGTGCAGGTTGGATGAACTGAGCAATCGCTTTTAGA43900.14138882474323758No Hit
GGTTACTATACACAACTGGCAAAACCTGAAAATGAAGAATTGGGGGGTGA43140.13894109110303574No Hit
ACCTTCTATAGAAAACAACATGTAAAGCAATATACAAAGAATAAATATAA42620.1372663259807924No Hit
CCCAATTATAAAGCACATGGACGCCTAGTTTCACTTTTTGTTTCTACACT42360.13642894341967068No Hit
CCACTCTATATCGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGA42230.13601025213910983No Hit
GAATGGTATAGTTGTATTTGAGAGTAGAAGTGGCTGATGATATTGTTTGT42140.13572038894487543No Hit
CGTACTTATAATTTTGAGGGTTTGGTCGAGTTGTTAAAAGCCAAGAAGAT41660.1341744519089585No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT41600.13398120977946887No Hit
CGTCGCTATAAGTTGCTTTGCAGTTGTTTTCAGAGATTTGAAAACGTTTG41420.133401483391No Hit
CGTGAATATACATTATAAGGTTTTGATTAATGTTTGTTTCACTGATTTAG41260.13288617104569433No Hit
AAGACTTATATGGGGGCCATTGTACAATGAAAGTGTGCTATATTAAAATT40870.13163009720401184No Hit
TCTTATTTGGCGGTGTAATCGTTGGAGCTCTTCTACTAATTCTGTTAGGT39770.1280873248300355No Hit
AACATTTATCTGCTTTTATAGAGTTTTAAATTTCGTTTTTTTTTTTCTTT39040.12573621225457846No Hit
GTGCGTTATAGGGACTGAATGGGAGAAAATATTTGTTACCGGCTTTGTTT38020.12245109605325497No Hit
TCCCACTATACTACCGGAGACGACGACGGTGACGGCTTTGAAGAAATTTA36660.11807094111815693No Hit
GGTTGATATAGGGACTGGGGATCAGCGGATGTTGCTTATAGGACTCCGCT36350.11707252344912723No Hit
TGATCATAAAGTACTTAGGTAAACAACGGAAAAATTTAGATTTGATAGAT36040.11607410578009754No Hit
CAACCTTATAATATATATGATATGTTAATTAGTTAAATTATTTAATCATT35300.1136907861830589No Hit
TTCCCTTATAGAAAACAATACAACAAATCATACACATCTCTCATAATTTA34990.11269236851402921No Hit
CCGGCGATTACTAGCGATTCCGGCTTCATGCAGGCGAGTTGCAGCCTGCA34580.11137188062918348No Hit
AGAGCACACGTCTGAACAAGAGCACACGTCTGAACAGCACACGTCTGAAC34260.1103412559385722No Hit
CAACGGGTTACCGAATTCAGTCAAACTCCGAATGCCAAAGACTTATCCAT33670.1084410416652576No Hit
ATTCGTTATAAATGCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCG33450.10773248719046233No Hit
GTCCCTTCATCATGTGAGCTTTCTTCAACTTTTTCACTGTCATCTGTCTT32690.10528475355026051No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)