FastQCFastQC Report
Wed 8 Feb 2023
HLWJFDRX2_n01_12R_2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWJFDRX2_n01_12R_2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4182701
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAAG87570.2093623235320909No Hit
GCGACCCCAGGTCAGGCGGGATTACCCGCTGAGTTTAAGCATATCAATAA62310.14897072489761998No Hit
GGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCGA56050.1340043192186102No Hit
CAAGGCTAAATACGGGCGAGAGACCGATAGCGAACAAGTACCGCGAGGTA50410.12052020930972593No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)