FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_4bw-2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_4bw-2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18821132
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG208380.11071597606350139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCG55100.032.1212044
CATGCGT57950.030.3628675
ATGCGTG59300.030.3114536
CTGACAT68200.027.8839861
TGCGTGC65400.027.3769877
TGCGAGT64900.027.00333411
GCCAGTA52250.026.1575161
GTGCGAG68950.025.51713810
TCGTATG13200.025.09004443
CTCGTAT12650.023.99917242
CGGGACT36200.022.119081
GAGTCGA81150.021.68103414
AGTCGAC83050.021.35117715
TTCGATC54350.020.7581252
TATGCCG16550.020.21963146
GGCTCAG48700.019.76394311
GTCCCTA49950.019.545518
CTATAAG71500.019.2515846
GTCGAAT15900.019.101751
ACATCCG70250.018.95638555
Produced by FastQC (version 0.11.2)