FastQCFastQC Report
Fri 6 Apr 2018
HLWCYBGX5_n01_ir64_4bw-2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHLWCYBGX5_n01_ir64_4bw-2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18144675
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT17400.021.6111342
TCGTATG19300.021.09234643
ACATGCG38850.018.116194
CGTATGC24400.017.2492544
ATGCGTG42450.017.148756
TATGCCG25350.016.87514546
ACGTCTG28250.016.85231815
CATGCGT42850.016.183525
CGCTATG50250.015.85896829
GTCGCTA51800.015.25122527
CTATAAG48600.015.04885446
CTTCCGA66100.014.7245431
TGCGAGT44850.014.69162811
CGCGAAT35850.014.4409671
TGCGTGC52100.014.3026027
TATAAGC50100.014.18512947
TATGCGG52400.014.1553155
GTCCCTA35400.014.033258
CTATGCG53300.013.98101754
TTCCGAT69650.013.8699282
Produced by FastQC (version 0.11.2)